caa a22 4500 463186818 CHVBK 20180406164856.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0532-1 doi (NATIONALLICENCE)springer-10.1007/s00726-007-0532-1 Enhancement of intestinal absorption of macromolecules by spermine in rats [Elektronische Daten] [Y. Sugita, K. Takao, Y. Toyama, A. Shirahata] Summary.: The aim of this study was to investigate the enhancing effect of polyamines on intestinal absorption of fluorescein isothiocyanate-labeled dextran (MW 4400, FD-4) in the in situ loop study and in vivo oral absorption study. Absorption of FD-4 from the jejunum was significantly enhanced by 5 mM spermine without serious membrane damage in the jejunum. An in vivo oral absorption study was also performed, and plasma FD-4 levels increased significantly after co-administration of 30 mM spermine. In the in vitro transport studies with Caco-2 cells, prolonged incubation with spermine resulted in a gradual decrease in transepithelial electrical resistance. This finding suggests that the absorption-enhancing mechanism of spermine partly includes opening the tight junctions of the epithelium via the paracellular route. These results indicate that excess oral ingestion of polyamines may have widespread health effects via the modulation of the intestinal epithelial barrier function. Springer-Verlag, 2007 Keywords: Polyamines - Fluorescein isothiocyanate-labeled dextran - Intestinal absorption - in situ loop - Oral absorption - Caco-2 cell monolayers nationallicence C10 : sodium caprate nationallicence C-CPE : C-terminal fragment of Clostridium perfringens enterotoxin nationallicence FD-4 : fluorescein isothiocyanate-labeled dextran 4400 nationallicence HBSS : Hanks balanced salt solution nationallicence HEPES : N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) nationallicence LDH : lactate dehydrogenase nationallicence MES : morpholinoethane sulfonic acid nationallicence TEER : transepithelial electrical resistance nationallicence Sugita Y. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut Takao K. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut Toyama Y. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut Shirahata A. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut Amino Acids Springer-Verlag 33/2(2007-08-01), 253-260 0939-4451 33:2<253 2007 33 726 https://doi.org/10.1007/s00726-007-0532-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0532-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugita Y. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut NATIONALLICENCE 700 1- Takao K. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut NATIONALLICENCE 700 1- Toyama Y. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut NATIONALLICENCE 700 1- Shirahata A. Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 253-260 0939-4451 33:2<253 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer