caa a22 4500 463186877 CHVBK 20180406164856.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00726-007-0513-4 doi (NATIONALLICENCE)springer-10.1007/s00726-007-0513-4 Spermine and spermidine mediate protection against oxidative damage caused by hydrogen peroxide [Elektronische Daten] [J. E. Rider, A. Hacker, C. A. Mackintosh, A. E. Pegg, P. M. Woster, R. A. Casero Jr] Summary.: The polyamines spermidine and spermine have been hypothesized to possess different functions in the protection of DNA from reactive oxygen species. The growth and survival of mouse fibroblasts unable to synthesize spermine were compared to their normal counterparts in their native and polyamine-depleted states in response to oxidative stress. The results of these studies suggest that when present at normal or supraphysiological concentrations, either spermidine or spermine can protect cells from reactive oxygen species. However, when polyamine pools are pharmacologically manipulated to produce cells with low levels of predominately spermine or spermidine, spermine appears to be more effective. Importantly, when cells are depleted of both glutathione and endogenous polyamines, they exhibit increased sensitivity to hydrogen peroxide as compared to glutathione depletion alone, suggesting that polyamines not only play a role in protecting cells from oxidative stress but this role is distinct from that played by glutathione. Springer-Verlag, 2007 Keywords: Polyamines - Spermine - Spermidine - Antioxidants - Hydrogen peroxide - Oxidative damage nationallicence BSO : DL-Buthionine-[S, R]-sulfoximine nationallicence CGP48664 : 4-amidinoindan-1-one 2′amidinhydrazone nationallicence DAPI : 4′,6′-diamidino-2-phenylindole hydrochloride nationallicence DFMO : difluoromethylornithine nationallicence FITC : fluorescein isothiocyanate nationallicence GSH : Glutathione nationallicence PBS : phosphate-buffered saline nationallicence ROS : reactive oxygen species nationallicence Rider J. E. Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA aut Hacker A. The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA aut Mackintosh C. A. Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, USA aut Pegg A. E. Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, USA aut Woster P. M. Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI, USA aut Casero Jr R. A. Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA aut Amino Acids Springer-Verlag 33/2(2007-08-01), 231-240 0939-4451 33:2<231 2007 33 726 https://doi.org/10.1007/s00726-007-0513-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00726-007-0513-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rider J. E. Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Hacker A. The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Mackintosh C. A. Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, USA aut NATIONALLICENCE 700 1- Pegg A. E. Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, USA aut NATIONALLICENCE 700 1- Woster P. M. Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI, USA aut NATIONALLICENCE 700 1- Casero Jr R. A. Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Amino Acids Springer-Verlag 33/2(2007-08-01), 231-240 0939-4451 33:2<231 2007 33 726 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer