caa a22 4500 463189094 CHVBK 20180406164903.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10973-006-8072-6 doi (NATIONALLICENCE)springer-10.1007/s10973-006-8072-6 Effectof ethanol on the thermal stability of human serum albumin [Elektronische Daten] [Anna Michnik, Zofia Drzazga] The effect of ethanol on human serum albumin stability in aqueous solution was studied by use of differential scanning calorimetry. A deconvolution of DSC traces in 2-state model with ΔC p=0 and ΔC p≠0 was performed and analysed to obtain information on the interaction of ethanol with different parts of albumin molecule both fatty acid containing and fatty acid free. The differences in ethanol binding affinity for both kinds of albumin were found. At very low concentrations ethanol was observed to be a stabilizer of the folded state of albumin contrary to the higher concentration where its binding to the unfolded protein predominates. Springer-Verlag, 2007 albumin-ethanol interactions nationallicence DSC nationallicence human serum albumin nationallicence thermal stability nationallicence Michnik Anna A. Chełkowski' Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland aut Drzazga Zofia A. Chełkowski' Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland aut Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers 88/2(2007-05-01), 449-454 1388-6150 88:2<449 2007 88 10973 https://doi.org/10.1007/s10973-006-8072-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-8072-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michnik Anna A. Chełkowski' Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland aut NATIONALLICENCE 700 1- Drzazga Zofia A. Chełkowski' Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers 88/2(2007-05-01), 449-454 1388-6150 88:2<449 2007 88 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer