caa a22 4500 463190912 CHVBK 20180406164908.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s10973-006-7918-2 doi (NATIONALLICENCE)springer-10.1007/s10973-006-7918-2 Microcalorimetry as a tool for Cr(VI) toxicity evaluation of human dermal fibroblasts [Elektronische Daten] [W. Liu, F. Chaspoul, D. Berge Lefranc, L. Decome, P. Gallice] Occupational exposure to Cr(VI) causes various effects including deep skin ulcerations. Its action mechanisms are not fully understood. In the present study, the evaluation of human dermal fibroblasts heat production was monitored, using microcalorimetry. as part of Cr(VI) toxicity. In control cells, normal heat production was 15±5 pW/cell. Regardless of the Cr(VI) concentration tested (0 to 500 μM), heat production was inhibited over time periods ranging from 3 to 25 h. These results could be correlated with cell mortality and the IC50 for Cr(VI) was 29±4 μM. In the WST-1 bioassay, the IC50 was 35±5 μM (no statistical difference). Thus, Cr(VI) altered the metabolism of the fibroblasts, and led to cellular death. Microcalorimetry can be a useful tool for determining the toxic effect of suspect compounds implicated in the occurrence of pathologies. Springer Science+Business Media LLC, 2007 cytotoxicity assay nationallicence fibroblasts nationallicence hexavalent chromium (Cr(VI) nationallicence microcalorimetry nationallicence Liu W. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut Chaspoul F. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut Berge Lefranc D. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut Decome L. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut Gallice P. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 89/1(2007-07-01), 21-24 1388-6150 89:1<21 2007 89 10973 https://doi.org/10.1007/s10973-006-7918-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-7918-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu W. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut NATIONALLICENCE 700 1- Chaspoul F. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut NATIONALLICENCE 700 1- Berge Lefranc D. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut NATIONALLICENCE 700 1- Decome L. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut NATIONALLICENCE 700 1- Gallice P. Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385, Marseille cedex 5, France aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 89/1(2007-07-01), 21-24 1388-6150 89:1<21 2007 89 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer