caa a22 4500 463191129 CHVBK 20180406164909.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10973-006-7598-y doi (NATIONALLICENCE)springer-10.1007/s10973-006-7598-y Thermal and FTIR investigation of freeze-dried protein-excipient mixtures [Elektronische Daten] [J. Lu, X. Wang, Y. Liu, C. Ching] The effect of excipients on the secondary structure of lyophilized proteins was studied through second-derivative Fourier transform infrared (FTIR) spectroscopic analysis. The glass transition temperature (T g), denaturation temperature (T d) and moisture content were determined by differential scanning calorimetry (DSC) and thermogravimetry (TG). T g, T d and the preservation of protein secondary structure were found to be dependent upon the type and amount of the excipient included in the formulation. Meanwhile, the lyophilized proteins easily adsorbed amounts of moisture during storage to reduce their T gs and stability. Springer Science+Business Media LLC, 2007 excipients nationallicence freeze-drying nationallicence proteins nationallicence secondary structure nationallicence stabilization nationallicence Lu J. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut Wang X. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut Liu Y. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut Ching C. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut Journal of Thermal Analysis and Calorimetry Springer US; http://www.springer-ny.com 89/3(2007-09-01), 913-919 1388-6150 89:3<913 2007 89 10973 https://doi.org/10.1007/s10973-006-7598-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-7598-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu J. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut NATIONALLICENCE 700 1- Wang X. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut NATIONALLICENCE 700 1- Liu Y. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut NATIONALLICENCE 700 1- Ching C. Division of Chemical and Biomolecular Engineering, Nanyang Technological University, Nanyang, Singapore aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Springer US; http://www.springer-ny.com 89/3(2007-09-01), 913-919 1388-6150 89:3<913 2007 89 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer