caa a22 4500 463191307 CHVBK 20180406164909.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10973-006-7611-5 doi (NATIONALLICENCE)springer-10.1007/s10973-006-7611-5 Microcalorimetric studies on the interactions of lanthanide ions with bovine serum albumin [Elektronische Daten] [X. Li, C. Wang, J. Li, Z. Wang] The interactions of lanthanide ions (Ln3+) with bovine serum albumin (BSA) under mimetic physiological conditions (310.15 K, pH 6.7, 0.1MNaCl) were studied by microcalorimetry. For the first time, based on Two Sets of Independent Sites Model, molar enthalpies (Δr H m1, Δr H m2) and coordination number (n 1, n 2) of the two sets of binding sites with different affinity were obtained directly from the microcalorimetric results. It was shown that the interactions are endothermic and entropy-driving processes. By combining with fluorescence spectroscopy, other thermodynamic parameters (Δr G m1, Δr S m1) were determined for high-affinity specific sites. Springer Science+Business Media LLC, 2007 bovine serum albumin (BSA) nationallicence fluorescence spectroscopy nationallicence lanthanide ions (Ln3+) nationallicence microcalorimetry nationallicence Li X. School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, People's Republic of China aut Wang C. College of Chemistry and Molecular Science, Wuhan University, 430072, Wuhan, People's Republic of China aut Li J. College of Chemistry and Molecular Science, Wuhan University, 430072, Wuhan, People's Republic of China aut Wang Z. College of Chemistry and Molecular Science, Wuhan University, 430072, Wuhan, People's Republic of China aut Journal of Thermal Analysis and Calorimetry Springer US; http://www.springer-ny.com 89/3(2007-09-01), 899-905 1388-6150 89:3<899 2007 89 10973 https://doi.org/10.1007/s10973-006-7611-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-7611-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li X. School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, 411201, Xiangtan, People's Republic of China aut NATIONALLICENCE 700 1- Wang C. College of Chemistry and Molecular Science, Wuhan University, 430072, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Li J. College of Chemistry and Molecular Science, Wuhan University, 430072, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Z. College of Chemistry and Molecular Science, Wuhan University, 430072, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Springer US; http://www.springer-ny.com 89/3(2007-09-01), 899-905 1388-6150 89:3<899 2007 89 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer