caa a22 4500 463192184 CHVBK 20180406164912.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s10973-006-7822-9 doi (NATIONALLICENCE)springer-10.1007/s10973-006-7822-9 Comparativestudy of thermal stability of healthy and focal segmental glomerulosclerosisplasma albumin [Elektronische Daten] [M. Bruschi, L. Musante, G. Ghiggeri, G. Rialdi, G. Candiano] The thermodynamic parameters calculated from measurements obtained by differential scanning calorimetry from healthy and focal segmental glomerulosclerosis albumin are reported. The same values were determined by fluorescence spectra and by the second derivative absorption spectra and they resulted in agreement with values obtained from the calorimetry technique. Nevertheless the unfolding mechanism seems to be completely altered when pathological albumin is compared with healthy albumin. The C p values measured by calorimetry show an increase with mild slope with healthy protein; on the contrary the slope intensely increase with pathological protein. Furthermore the λmax of this molecule is lower and drastically decrease with the increase of temperature when compared with healthy one. Therefore the modification of cys 34 on pathological albumin is supposed to cause an alteration of the structure, the swelling and the unfolding mechanism. Springer-Verlag, 2007 albumin nationallicence differential scanning calorimetry (DSC) nationallicence focal segmental glomerulosclerosis (FSGS) nationallicence intrinsic fluorescence nationallicence plasma nationallicence second derivative UV spectrum nationallicence Bruschi M. RenalChild Foundation, Genoa, Italy aut Musante L. RenalChild Foundation, Genoa, Italy aut Ghiggeri G. Laboratory on Pathophysiology of Uremia, G. Gaslini Children Hospital, Genoa, Italy aut Rialdi G. RenalChild Foundation, Genoa, Italy aut Candiano G. Laboratory on Pathophysiology of Uremia, G. Gaslini Children Hospital, Genoa, Italy aut Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers 87/1(2007-01-01), 27-31 1388-6150 87:1<27 2007 87 10973 https://doi.org/10.1007/s10973-006-7822-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-7822-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bruschi M. RenalChild Foundation, Genoa, Italy aut NATIONALLICENCE 700 1- Musante L. RenalChild Foundation, Genoa, Italy aut NATIONALLICENCE 700 1- Ghiggeri G. Laboratory on Pathophysiology of Uremia, G. Gaslini Children Hospital, Genoa, Italy aut NATIONALLICENCE 700 1- Rialdi G. RenalChild Foundation, Genoa, Italy aut NATIONALLICENCE 700 1- Candiano G. Laboratory on Pathophysiology of Uremia, G. Gaslini Children Hospital, Genoa, Italy aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers 87/1(2007-01-01), 27-31 1388-6150 87:1<27 2007 87 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer