caa a22 4500 46319232X CHVBK 20180406164912.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s10973-006-7825-6 doi (NATIONALLICENCE)springer-10.1007/s10973-006-7825-6 Michnik Anna A. Chełkowski' Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland aut DSCstudy of the association of ethanol with human serum albumin [Elektronische Daten] [Anna Michnik] Human serum albumin unfolding in ethanol/water mixtures was studied by use of differential scanning calorimetry. Ethanol-induced changes in DSC curves of defatted and non-defatted albumin were markedly different. In the presence of ethanol, bimodal denaturation transition for fatty acid free albumin was observed while that for albumin containing endogenous fatty acids was single and more sharpen than in aqueous solution. Ethanol was found to decrease the thermal stability of albumin due to the binding to the unfolded state to a higher degree than to the native state, thus favouring unfolding. The binding with different affinities has been suggested depending on ethanol concentration range. Springer-Verlag, 2007 DSC nationallicence ethanol binding nationallicence human serum albumin nationallicence thermal stability nationallicence Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers 87/1(2007-01-01), 91-96 1388-6150 87:1<91 2007 87 10973 https://doi.org/10.1007/s10973-006-7825-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-7825-6 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Michnik Anna A. Chełkowski' Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Kluwer Academic Publishers 87/1(2007-01-01), 91-96 1388-6150 87:1<91 2007 87 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer