caa a22 4500 463193520 CHVBK 20180406164915.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s10973-005-6906-2 doi (NATIONALLICENCE)springer-10.1007/s10973-005-6906-2 Interactionof a homologous series of n -alkyl trimethylammonium bromides with eggwhite lysozyme [Elektronische Daten] Microcalorimetricand spectroscopic study [A. Bordbar, R. Hosseinzadeh, M. Norozi] The interaction of a series of n-alkyl trimethyl ammonium bromides (C12, C14 and C16) with egg white lysozyme have been studied using fluorescence and UV-Vis spectroscopies and isothermal titration calorimetry (ITC). The trend of variation of molar absorptivity at 281 nm, quantum yields (λex=281 nm) and heat of interaction with respect to surfactant concentration, were measured. The spectrophotometric results show that the hydrophobic interactions have a major role in denaturation mechanism and it would be increased with increasing in hydrocarbon tail length of surfactant. The ITC results indicated the two-step mechanism for unfolding of lysozyme due to its interaction with surfactants. Springer-Verlag, 2007 absorption spectroscopy nationallicence cationic surfactants nationallicence fluorescence spectroscopy nationallicence ITC nationallicence lysozyme nationallicence unfolding nationallicence Bordbar A. Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, 81746-73441, Isfahan, Iran aut Hosseinzadeh R. Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, 81746-73441, Isfahan, Iran aut Norozi M. Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, 81746-73441, Isfahan, Iran aut Journal of Thermal Analysis and Calorimetry Springer Netherlands 87/2(2007-01-01), 453-456 1388-6150 87:2<453 2007 87 10973 https://doi.org/10.1007/s10973-005-6906-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-005-6906-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bordbar A. Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, 81746-73441, Isfahan, Iran aut NATIONALLICENCE 700 1- Hosseinzadeh R. Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, 81746-73441, Isfahan, Iran aut NATIONALLICENCE 700 1- Norozi M. Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, 81746-73441, Isfahan, Iran aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Springer Netherlands 87/2(2007-01-01), 453-456 1388-6150 87:2<453 2007 87 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer