caa a22 4500 463193628 CHVBK 20180406164916.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s10973-006-7880-z doi (NATIONALLICENCE)springer-10.1007/s10973-006-7880-z Structureof starches extracted from near-isogenic wheat lines [Elektronische Daten] Part 2. Molecular organization of amylopectin clusters [S. Kozlov, A. Krivandin, Olga Shatalova, T. Noda, E. Bertoft, J. Fornal, V. Yuryev] High-sensitivity differential scanning calorimetry (HSDSC) and small-angle X-ray scattering (SAXS) were used to investigate the structural characteristics of starch granules with different amylose content extracted from near-isogenic wheat lines with different combinations of active granule-bound starch synthase (GBSS I) isoforms. Paracrystalline diffraction model, ‘two-state' model of starch melting and other physico-chemical approaches were used to estimate the sizes of amylopectin clusters, the thicknesses of crystalline lamellae and the structure of amylopectin defects for investigated wheat starches. The joint analysis of SAXS and DSC data has shown that the size of amylopectin cluster, the thickness of crystalline lamellae and the structure of amylopectin defects do not depend on the differences in combinations of active GBSS I isozymes. The data obtained supposed that the amylopectin cluster size and the thickness of crystalline lamellae are, generally, the universal structural parameters for wheat starches. Additionally, the data obtained suggest that increase of amylose content is accompanied by accumulation of both amylose tie-chains, located as defects in crystalline lamellae, and amylose chains oriented transversely to the lamella stack within amorphous lamellae. Disordered ends of amylopectin double helices and/or pre-existing double helices not participating in formation of crystals are considered as amylopectin defects arranged in crystalline lamellae. The relationship between structure of wheat starches extracted from near-isogenic lines and their thermodynamic properties was recognized. Springer-Verlag, 2006 amylose and amylopectin localization nationallicence cluster and lamellar structure nationallicence GBSS I nationallicence HSDSC nationallicence native and annealed wheat starches nationallicence SAXS nationallicence Kozlov S. Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut Krivandin A. Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut Shatalova Olga Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut Noda T. Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, NARO Shinsei, 082-0071, Memuro Kasai, Hokkaido, Japan aut Bertoft E. Department of Biochemistry and Pharmacy, Abo Akademi University, BioCity, P.O. Box 66, 20521, Turku, Finland aut Fornal J. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10 Tuwina Str., 10-747, Olsztyn, Poland aut Yuryev V. Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut Journal of Thermal Analysis and Calorimetry Springer Netherlands 87/2(2007-01-01), 575-584 1388-6150 87:2<575 2007 87 10973 https://doi.org/10.1007/s10973-006-7880-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10973-006-7880-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kozlov S. Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Krivandin A. Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Shatalova Olga Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Noda T. Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, NARO Shinsei, 082-0071, Memuro Kasai, Hokkaido, Japan aut NATIONALLICENCE 700 1- Bertoft E. Department of Biochemistry and Pharmacy, Abo Akademi University, BioCity, P.O. Box 66, 20521, Turku, Finland aut NATIONALLICENCE 700 1- Fornal J. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10 Tuwina Str., 10-747, Olsztyn, Poland aut NATIONALLICENCE 700 1- Yuryev V. Institute of Biochemical Physics, Russian Academy of Sciences, 4, str. Kosygina, 119991, Moscow, Russia aut NATIONALLICENCE 773 0- Journal of Thermal Analysis and Calorimetry Springer Netherlands 87/2(2007-01-01), 575-584 1388-6150 87:2<575 2007 87 10973 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer