caa a22 4500 463194225 CHVBK 20180406164918.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s00420-006-0128-1 doi (NATIONALLICENCE)springer-10.1007/s00420-006-0128-1 8-Hydroxydeoxyguanosine levels in human leukocyte and urine according to exposure to organophosphorus pesticides and paraoxonase 1 genotype [Elektronische Daten] [Chul-Ho Lee, Michihiro Kamijima, Heon Kim, Eiji Shibata, Jun Ueyama, Takayoshi Suzuki, Kenji Takagi, Isao Saito, Masahiro Gotoh, Hatsuki Hibi, Hisao Naito, Tamie Nakajima] Objective: In order to investigate a role of paraoxonase 1 (PON1) polymorphism in organophosphorus (OP)-induced 8-hydroxydeoxyguanosine (8-OHdG) levels, urinary metabolites of OP, PON1 genotypes, and 8-OHdG levels in leukocyte and urine were measured in OP indoor insecticide sprayers and controls in summer and winter. Methods: The study population contained 18 male sprayers and age-matched 18 male controls. Sprayers were primarily exposed to OP insecticides (mainly fenitrothion, dichlorvos, chlorpyrifos, and diazinon), and partially to pyrethroids (mainly permethrin) and carbamates (mainly propoxur). Urinary metabolites of OP were measured by gas chromatography-mass spectrometry. 8-OHdG levels in leukocyte and urine were measured by ELISA kit. PON1 genotype was identified using allele-specific fluorogenic TaqMan probes. Results: The mean concentrations of urinary dimethyl phosphate (DMP) and total dialkyl phosphates (DAP) in summer and those of 8-OHdG in summer and winter were significantly higher in OP sprayers than controls. This resulted in a significant positive correlation between 8-OHdG levels and urinary DMP or DAP, suggesting a correlation between OP metabolites and production of oxidative stress. Of PON1 genotypes, incidences of Q/Q, Q/R, and R/R types were 17, 39, and 44% in OP sprayers and controls, respectively. Although PON1 polymorphism did not contribute to the leukocyte and urinary 8-OHdG levels, the urinary OP metabolite concentrations in summer showed a significant decrease as the number Q allele decreased. Conclusion: These results indicate that an increase in OP metabolites is associated with a high level of oxidative stress in OP sprayers, although the contribution of the PON1 polymorphism to the metabolism of OP is still unclear. Springer-Verlag, 2006 8-Hydroxydeoxyguanosine nationallicence Organophosphorus compound nationallicence Paraoxonase 1 nationallicence Polymorphism nationallicence OP : Organophosphorus nationallicence PON : Paraoxonase nationallicence DMP : Dimethyl phosphate nationallicence DEP : Diethyl phosphate nationallicence DMTP : Dimethylthio phosphate nationallicence DETP : Diethylthio phosphate nationallicence DAP : Dialkyl phosphate nationallicence ROS : Reactive oxygen species nationallicence H2O2 : Hydrogen peroxide nationallicence O 2 − : Superoxide nationallicence HO : Hydroxyl radical nationallicence 8-OHdG : 8-Hydroxydeoxyguanosine nationallicence Lee Chul-Ho Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Kamijima Michihiro Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Kim Heon Department of Preventive Medicine, College of Medicine, Chungbuk National University, 361-763, Cheongju, South Korea aut Shibata Eiji Department of Health and Psychosocial Medicine, Aichi Medical University School of Medicine, 480-1195, Aichi, Japan aut Ueyama Jun Department of Clinical Laboratory, Nagoya University Hospital, 466-8550, Nagoya, Japan aut Suzuki Takayoshi Department of Medical Technology, Nagoya University School of Health Sciences, 461-8673, Nagoya, Japan aut Takagi Kenji Department of Medical Technology, Nagoya University School of Health Sciences, 461-8673, Nagoya, Japan aut Saito Isao Food Safety and Quality Research Center, Tokai Coop Federation, 480-1103, Aichi, Japan aut Gotoh Masahiro Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Hibi Hatsuki Kyoritsu General Hospital, 456-0058, Nagoya, Japan aut Naito Hisao Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut Nakajima Tamie Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut International Archives of Occupational and Environmental Health Springer-Verlag 80/3(2007-01-01), 217-227 0340-0131 80:3<217 2007 80 420 https://doi.org/10.1007/s00420-006-0128-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00420-006-0128-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Chul-Ho Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Kamijima Michihiro Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Kim Heon Department of Preventive Medicine, College of Medicine, Chungbuk National University, 361-763, Cheongju, South Korea aut NATIONALLICENCE 700 1- Shibata Eiji Department of Health and Psychosocial Medicine, Aichi Medical University School of Medicine, 480-1195, Aichi, Japan aut NATIONALLICENCE 700 1- Ueyama Jun Department of Clinical Laboratory, Nagoya University Hospital, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Suzuki Takayoshi Department of Medical Technology, Nagoya University School of Health Sciences, 461-8673, Nagoya, Japan aut NATIONALLICENCE 700 1- Takagi Kenji Department of Medical Technology, Nagoya University School of Health Sciences, 461-8673, Nagoya, Japan aut NATIONALLICENCE 700 1- Saito Isao Food Safety and Quality Research Center, Tokai Coop Federation, 480-1103, Aichi, Japan aut NATIONALLICENCE 700 1- Gotoh Masahiro Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Hibi Hatsuki Kyoritsu General Hospital, 456-0058, Nagoya, Japan aut NATIONALLICENCE 700 1- Naito Hisao Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Nakajima Tamie Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumaicho, Showa-ku, 466-8550, Nagoya, Aichi, Japan aut NATIONALLICENCE 773 0- International Archives of Occupational and Environmental Health Springer-Verlag 80/3(2007-01-01), 217-227 0340-0131 80:3<217 2007 80 420 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer