caa a22 4500 463194616 CHVBK 20180406164919.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00420-006-0130-7 doi (NATIONALLICENCE)springer-10.1007/s00420-006-0130-7 Micronucleus frequency in peripheral blood lymphocytes and buccal mucosa cells of copper smelter workers, with special regard to arsenic exposure [Elektronische Daten] [D. Lewińska, J. Palus, M. Stępnik, E. Dziubałtowska, J. Beck, K. Rydzyński, A. Natarajan, R. Nilsson] Occupational exposure in copper smelters may produce various adverse health effects including cancer which, according to available epidemiologic data, is associated mainly with exposure to arsenic. Despite a number of well-documented studies reporting an increased risk of cancer among copper smelters workers, the data on genotoxic effects in this industry are scarce. In view of the above, an assessment of micronuclei (MN) frequency in peripheral blood lymphocytes and buccal epithelial cells from copper smelter workers was undertaken. Additionally, the clastogenic/aneugenic effect in lymphocytes was assessed with the fluorescence in situ hybridization (FISH). The study was conducted in three copper smelters in southwestern Poland. The subjects (n=72) were enrolled among male workers at departments where As concentration in the air was up to at 80μg/m3. Exposure was assessed by measurement of arsenic concentration in urine and toenail samples. The control group (n=83) was recruited from healthy male individuals living in central Poland who did not report any exposure to known genotoxins. The results of our study showed a significant increase in MN frequency in peripheral blood lymphocytes and in buccal epithelial cells of smelter workers, compared to the controls (7.96±4.28 vs. 3.47±1.70 and 0.98±0.76 vs. 0.50±0.52, respectively). The FISH technique revealed the presence of clastogenic and aneugenic effects in peripheral blood lymphocytes in both groups. The clastogenic effect was slightly more pronounced in the smelter workers; however, the difference was not statistically significant. The mean arsenic concentrations in urine (total arsenic species) and in toenail samples in the exposed group were 54.04±42.26μg/l and 7.63±7.24μg/g, respectively, being significantly different from control group 11.01±10.84μg/l and 0.51±0.05μg/g. No correlation between As content in urine or toenail samples and the genotoxic effect was found under study. Springer-Verlag, 2007 Arsenic nationallicence Occupational exposure nationallicence Micronucleus test nationallicence Fluorescence in situ hybridization (FISH) nationallicence Lewińska D. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut Palus J. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut Stępnik M. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut Dziubałtowska E. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut Beck J. Abteilung fur Allgemeine Hygiene und Umweltmedizin, Georg-August-Universitat, Gottingen, Germany aut Rydzyński K. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut Natarajan A. Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands aut Nilsson R. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut International Archives of Occupational and Environmental Health Springer-Verlag 80/5(2007-04-01), 371-380 0340-0131 80:5<371 2007 80 420 https://doi.org/10.1007/s00420-006-0130-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00420-006-0130-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lewińska D. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut NATIONALLICENCE 700 1- Palus J. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut NATIONALLICENCE 700 1- Stępnik M. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut NATIONALLICENCE 700 1- Dziubałtowska E. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut NATIONALLICENCE 700 1- Beck J. Abteilung fur Allgemeine Hygiene und Umweltmedizin, Georg-August-Universitat, Gottingen, Germany aut NATIONALLICENCE 700 1- Rydzyński K. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut NATIONALLICENCE 700 1- Natarajan A. Department of Toxicogenetics, Leiden University Medical Center, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Nilsson R. Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, 8 Teresy St., 90-950, Lodz, Poland aut NATIONALLICENCE 773 0- International Archives of Occupational and Environmental Health Springer-Verlag 80/5(2007-04-01), 371-380 0340-0131 80:5<371 2007 80 420 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer