caa a22 4500 463195205 CHVBK 20180406164920.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10669-007-9078-8 doi (NATIONALLICENCE)springer-10.1007/s10669-007-9078-8 Effects of invitro exposure to GSM900 electromagnetic field on human erythrocytes [Elektronische Daten] [M. Kouzmanova, G. Atanasova, N. Atanasov, S. Tasheva] High frequency electromagnetic fields (EMF) are widely used for transmitting of radio and TV signals, in wireless communications, etc. A huge number of people are exposed so the possible risk to human health from telecommunication technologies could be significant even if biological effects are slight. The study of the biological effects of RF EM radiation could contribute to better understanding of the possible health hazards. The levels of released hemoglobin serve as an indicator of hemolysis, caused by increased membrane fragility. This study was designed to investigate the alterations in hemoglobin release after invitro exposure of human erythrocytes to GSM900 electromagnetic field. Erythrocyte suspensions with two different cell concentrations (hematocrit 20% and 40%) were exposed to EM radiation from GSM mobile phone (carrier frequency 902MHz, 2W output power in pulse) for 20min in two different positions in relation to telephone antenna: Position 1 is in the centre of the major lobe of the azimuth antenna pattern and Position 2 is between major and back lobes. Alterations in hemolysis were registered 0, 10, 20, 30, 40, 50 and 60min after the treatment. Hemolysis was determined by measuring the absorbance of hemoglobin at 413nm in the supernatant obtained by centrifuging the suspensions. Hemolysis was expressed as hemoglobin concentration. Our data indicated decrease in the hemoglobin level in irradiated suspensions. The GSM900 EMF exposure probably stabilized erythrocyte membrane and caused reduction in the hemolysis depending on the EMF parameters, on the suspension water content (hematocrit) and on the time elapsed after irradiation. Springer Science+Business Media, LLC, 2007 Erthrocyte membrane nationallicence Hemolysis nationallicence Mobile phones nationallicence 900 MHz electromagnetic field nationallicence Kouzmanova M. Department of Biophysics and Radiobiology, Biological Faculty, Sofia University, 8 Dragan Tzankov blvd., 1164, Sofia, Bulgaria aut Atanasova G. Department of Telecommunication Technologies, Higher College of Telecommunications and Posts, Sofia, Bulgaria aut Atanasov N. Department of Wireless Communications and Broadcasting, Higher College of Telecommunications and Posts, 1 Acad. Stefan Mladenov str., 1700, Sofia, Bulgaria aut Tasheva S. Department of Biophysics and Radiobiology, Biological Faculty, Sofia University, 8 Dragan Tzankov blvd., 1164, Sofia, Bulgaria aut The Environmentalist Springer US; http://www.springer-ny.com 27/4(2007-12-01), 423-428 0251-1088 27:4<423 2007 27 10669 https://doi.org/10.1007/s10669-007-9078-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10669-007-9078-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kouzmanova M. Department of Biophysics and Radiobiology, Biological Faculty, Sofia University, 8 Dragan Tzankov blvd., 1164, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Atanasova G. Department of Telecommunication Technologies, Higher College of Telecommunications and Posts, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Atanasov N. Department of Wireless Communications and Broadcasting, Higher College of Telecommunications and Posts, 1 Acad. Stefan Mladenov str., 1700, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Tasheva S. Department of Biophysics and Radiobiology, Biological Faculty, Sofia University, 8 Dragan Tzankov blvd., 1164, Sofia, Bulgaria aut NATIONALLICENCE 773 0- The Environmentalist Springer US; http://www.springer-ny.com 27/4(2007-12-01), 423-428 0251-1088 27:4<423 2007 27 10669 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer