caa a22 4500 463199901 CHVBK 20180405153109.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s00276-007-0225-1 doi (NATIONALLICENCE)springer-10.1007/s00276-007-0225-1 Qualitative comparison of anatomical microdissection, Sihler's staining and computerized reconstruction methods for visualizing intramuscular nerve branches [Elektronische Daten] [Nadir Gülekon, Tuncay Peker, Hasan Turgut, Afitap Anıl, Mustafa Karaköse] Stating backround: This study was designed to examine the entire intramuscular nerve distribution pattern of various human skeletal muscles in fetuses. Methods: In the present study rhomboid major, trapezius, long head of the biceps femoris and masseter muscles were investigated in five 18weeks old fetal cadavers. Anatomical microdissection was applied to one fetal cadaver. In two fetuses, the extramuscular (main), major and minor nerve branches, and anastomosis were examined using Sihler's staining and labeling. In the remaining two fetuses, consecutive slices with 0.5mm interval and 5μm thickness were obtained from each skeletal muscle. These slices were stained with S100 for the demonstration of the nerve fibers and thereafter 3D reconstruction images were constituted using PC software. Results: Anatomical microdissection, Sihler's staining and computerized reconstruction methods were compared to demonstrate the intramuscular nerve distribution pattern. Demonstration of the intramuscular minor nerve branches and anastomosis showed difficulties in anatomical dissected specimens when compared with three-dimensionally reconstructed images and specimens obtained with Sihler's staining technique. Nevertheless, anatomical dissection is a simple method whereas Sihler's technique and computer aided 3D reconstruction are complex methods and take a long time to complete. Conclusion: The obtained information exposed that staining technique and the 3D reconstructions appeared to provide better results than did anatomical dissection. Springer-Verlag, 2007 Anatomical dissection nationallicence Staining method nationallicence 3D reconstruction nationallicence Intramuscular innervation nationallicence Gülekon Nadir Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut Peker Tuncay Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut Turgut Hasan Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut Anıl Afitap Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut Karaköse Mustafa Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut Surgical and Radiologic Anatomy Springer-Verlag 29/5(2007-06-01), 373-378 0930-1038 29:5<373 2007 29 276 https://doi.org/10.1007/s00276-007-0225-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00276-007-0225-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gülekon Nadir Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut NATIONALLICENCE 700 1- Peker Tuncay Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut NATIONALLICENCE 700 1- Turgut Hasan Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut NATIONALLICENCE 700 1- Anıl Afitap Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut NATIONALLICENCE 700 1- Karaköse Mustafa Department of Anatomy, Gazi University Faculty of Medicine, 06500, Beşevler, Ankara, Turkey aut NATIONALLICENCE 773 0- Surgical and Radiologic Anatomy Springer-Verlag 29/5(2007-06-01), 373-378 0930-1038 29:5<373 2007 29 276 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer