caa a22 4500 463208102 CHVBK 20180405153132.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s10863-006-9064-8 doi (NATIONALLICENCE)springer-10.1007/s10863-006-9064-8 Hilf Russell Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, 14642, Rochester, NY, USA aut Mitochondria are targets of photodynamic therapy [Elektronische Daten] [Russell Hilf] Photodynamic Therapy (PDT) is an evolving cancer treatment that depends on three known and variable components: photosensitizer, light and oxygen. Optimization of these variables yields reactive oxygen species, mainly singlet oxygen, that damage cellular components leading to cytotoxicity. Our research has demonstrated that porphyrin sensitizers, in particular, significantly inhibit the inner mitochondrial membrane enzymes cytochrome c oxidase and F 0 F 1 ATP synthase. These results were obtained from an in vivo-in vitro experimental protocol that exposes sensitizers to metabolic and pharmacokinetic events. The resulting inhibition of oxidative phosphorylation was expected to reduce ATP levels, which were quantitated in cells and were confirmed by 31P-NMR spectroscopy of tumors in situ in animals treated with PDT. Based on these findings, and more recent investigations of apoptosis, there is little doubt that mitochondria are critical targets in the actions of PDT. Springer Science+Business Media, LLC, 2007 Photodynamic therapy nationallicence Mitochondrial targets nationallicence Enzyme inhibition nationallicence ATP levels nationallicence Tumor cytotoxicity nationallicence Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/1(2007-02-01), 85-89 0145-479X 39:1<85 2007 39 10863 https://doi.org/10.1007/s10863-006-9064-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-006-9064-8 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Hilf Russell Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, 14642, Rochester, NY, USA aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/1(2007-02-01), 85-89 0145-479X 39:1<85 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer