caa a22 4500 463208110 CHVBK 20180405153132.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s10863-006-9068-4 doi (NATIONALLICENCE)springer-10.1007/s10863-006-9068-4 Synchronization modulation of Na/K pump molecules can hyperpolarize the membrane resting potential in intact fibers [Elektronische Daten] [Wei Chen, Robin Dando] Previously, we have theoretically studied the possibility of electrical rhythmic entrainment of carrier-mediated ion transporters, and experimentally realized synchronization and acceleration of the Na/K pumping rate in the cell membrane of skeletal muscle fibers by a specially designed synchronization modulation electric field. In these studies we either used cut fibers under a voltage clamp or intact fibers, but in the presence of ion channels blockers. A question remained as to whether the field-induced activation observed in the pump molecules could effectively increase the intracellular ionic concentration and the membrane potential at physiological conditions. In this paper, we studied the effects of the field on intact fibers without any channel blockers. We monitored the field-induced changes in the ionic concentration gradient across the cell membrane and the membrane potential non-invasively by using a fluorescent probe and confocal microscopic imaging techniques. The results clearly show that the entrainment of the pump molecules by the synchronization modulation electric field can effectively increase the ionic concentration gradient, and hence, hyperpolarize the membrane potential. Springer Science+Business Media, LLC, 2007 Na/K pump molecules nationallicence Electric field nationallicence Synchronization nationallicence Modulation nationallicence Skeletal muscles nationallicence Intact fibers nationallicence Chen Wei Cellular and Molecular Biophysics, University of South Florida, 33620, Tampa, FL, USA aut Dando Robin Cellular and Molecular Biophysics, University of South Florida, 33620, Tampa, FL, USA aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/1(2007-02-01), 117-126 0145-479X 39:1<117 2007 39 10863 https://doi.org/10.1007/s10863-006-9068-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-006-9068-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Wei Cellular and Molecular Biophysics, University of South Florida, 33620, Tampa, FL, USA aut NATIONALLICENCE 700 1- Dando Robin Cellular and Molecular Biophysics, University of South Florida, 33620, Tampa, FL, USA aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/1(2007-02-01), 117-126 0145-479X 39:1<117 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer