caa a22 4500 463208145 CHVBK 20180405153132.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s10863-006-9067-5 doi (NATIONALLICENCE)springer-10.1007/s10863-006-9067-5 Fatty acid efficiency profile in uncoupling of Acanthamoeba castellanii mitochondria [Elektronische Daten] [Aleksandra Swida, Małgorzata Czarna, Andrzej Woyda-Płoszczyca, Anna Kicinska, Francis Sluse, Wieslawa Jarmuszkiewicz] A profile of free fatty acid (FFA) specificity in Acanthamoeba castellanii mitochondrial uncoupling is described. The FFA uncoupling specificity was observed as different abilities to stimulate resting respiration, to decrease resting membrane potential, and to decrease oxidative phosphorylation efficiency. Tested unsaturated FFA (C18-20) were more effective as uncouplers and protonophores when compared to tested saturated FFA (C8-18), with palmitic acid (C16:0) as the most active. As FFA efficiency in mitochondrial uncoupling is related to physiological changes of fatty acid composition (and thereby FFA availability) during growth of amoeba cells, it could be a way to regulate the activity of an uncoupling protein and thereby the efficiency of oxidative phosphorylation during a cell life of this unicellular organism. Springer Science+Business Media, LLC, 2007 Fatty acids nationallicence Uncoupling nationallicence Uncoupling protein nationallicence Mitochondria nationallicence Acanthamoeba castellanii nationallicence FFA: : free fatty acids nationallicence UCP: : uncoupling protein nationallicence AcUCP: : uncoupling protein of Acanthamoeba castellanii nationallicence Δψ: : mitochondrial membrane potential nationallicence ΔμH+: : proton electrochemical gradient nationallicence FCCP: : p-trifluoromethoxyphenylhydrazone nationallicence Swida Aleksandra Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Czarna Małgorzata Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Woyda-Płoszczyca Andrzej Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Kicinska Anna Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Sluse Francis Laboratory of Bioenergetics and Molecular Physiology, Liege University, B-4000, Sart Tilman, Liege, Belgium aut Jarmuszkiewicz Wieslawa Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/1(2007-02-01), 109-115 0145-479X 39:1<109 2007 39 10863 https://doi.org/10.1007/s10863-006-9067-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-006-9067-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Swida Aleksandra Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Czarna Małgorzata Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Woyda-Płoszczyca Andrzej Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Kicinska Anna Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Sluse Francis Laboratory of Bioenergetics and Molecular Physiology, Liege University, B-4000, Sart Tilman, Liege, Belgium aut NATIONALLICENCE 700 1- Jarmuszkiewicz Wieslawa Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/1(2007-02-01), 109-115 0145-479X 39:1<109 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer