caa a22 4500 463208285 CHVBK 20180405153132.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10863-007-9100-3 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9100-3 Sodium pump localization in epithelia [Elektronische Daten] [Jason Bystriansky, Jack Kaplan] In epithelial cells, the sodium pump, in coordination with several other ion transporting proteins and channels, acts to regulate directional water and ion flux across the epithelial barrier. This function is dependant on the polarized localization of the sodium pump to a single plasma membrane domain. In most epithelial cell types the sodium pump is found in an exclusively basolateral position. Despite the clear importance of maintaining a polarized distribution of the sodium pump, surprisingly littleis known about the specific mechanisms responsible for the targeting and trafficking of the sodium pump to the basolateral surface. We briefly discuss our current understanding of factors which may act to regulate the cellular distribution of the sodium pump, including the potential role of the sodium pump β-subunit. Several previous, studies have suggested that the expression of the β2 isoform (instead of β1) may cause the apical localization of the sodium pump. This appeared to be confirmed by Wilson et al. Am J Pathol, 156: 253-268, 2000 who found that MDCK cells stably transfected with the β2 subunit express the sodium pump at the apical surface. However, careful examination by Laughery et al.,Am J Physiol, 292: F1718-F1725, 2007, showed that the apical targeting of the pump was caused by the presence of butyrate in the cell growth media and was not due to the presence of the β2 isoform. These findings are discussed below, along with potential explanations as to how butyrate may influence the polarity of the sodium pump in epithelial cells. Springer Science+Business Media, LLC, 2007 Sodium pump nationallicence Epithelia nationallicence Ion transport nationallicence Membrane nationallicence Cell polarity nationallicence Trafficking nationallicence Butyrate nationallicence MDCK nationallicence Bystriansky Jason Department of Biochemistry & Molecular Genetics, University of Illinois at Chicago, Molecular Biology Research Building, 900 S Ashland Avenue, 60607, Chicago, IL, USA aut Kaplan Jack Department of Biochemistry & Molecular Genetics, University of Illinois at Chicago, Molecular Biology Research Building, 900 S Ashland Avenue, 60607, Chicago, IL, USA aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/5-6(2007-12-01), 373-378 0145-479X 39:5-6<373 2007 39 10863 https://doi.org/10.1007/s10863-007-9100-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9100-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bystriansky Jason Department of Biochemistry & Molecular Genetics, University of Illinois at Chicago, Molecular Biology Research Building, 900 S Ashland Avenue, 60607, Chicago, IL, USA aut NATIONALLICENCE 700 1- Kaplan Jack Department of Biochemistry & Molecular Genetics, University of Illinois at Chicago, Molecular Biology Research Building, 900 S Ashland Avenue, 60607, Chicago, IL, USA aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/5-6(2007-12-01), 373-378 0145-479X 39:5-6<373 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer