caa a22 4500 463208366 CHVBK 20180405153132.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10863-007-9114-x doi (NATIONALLICENCE)springer-10.1007/s10863-007-9114-x Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes [Elektronische Daten] [Justin York, David Spetzler, Tassilo Hornung, Robert Ishmukhametov, James Martin, Wayne Frasch] The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the γ-subunit was quantitated. Individual F1 molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1 molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was ∼50, ∼25, and ∼4% respectively. In some experiments, the fraction of molecules observed to rotate in the presence of GTP was as high as 65%. These data indicate that rotational measurements made using gold nanorods provide information of the F1-ATPase mechanism that is representative of the characteristics of the enzyme population as a whole. Springer Science+Business Media, LLC, 2007 F1-ATPase nationallicence Gold nanorods nationallicence Plasmon resonance nationallicence Molecular motors nationallicence Single molecule microscopy nationallicence York Justin School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut Spetzler David School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut Hornung Tassilo School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut Ishmukhametov Robert School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut Martin James School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut Frasch Wayne School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/5-6(2007-12-01), 435-439 0145-479X 39:5-6<435 2007 39 10863 https://doi.org/10.1007/s10863-007-9114-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9114-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- York Justin School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut NATIONALLICENCE 700 1- Spetzler David School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut NATIONALLICENCE 700 1- Hornung Tassilo School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut NATIONALLICENCE 700 1- Ishmukhametov Robert School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut NATIONALLICENCE 700 1- Martin James School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut NATIONALLICENCE 700 1- Frasch Wayne School of Life Sciences, Arizona State University, P.O. Box 874501, 85287-4501, Tempe, AZ, USA aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/5-6(2007-12-01), 435-439 0145-479X 39:5-6<435 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer