caa a22 4500 46320851X CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10863-007-9096-8 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9096-8 Entrainment of Na/K pumps by a synchronization modulation electric field [Elektronische Daten] [Wei Chen, Zhongsheng Zhang, Feiran Huang] We studied entrainment of the catalytic cycle of the Na/K pumps by an imposed external AC electric field. Our results show that a well designed dichotomous oscillating electric field with a frequency close to the pumps' natural turnover rate can synchronize the pump molecules. Characteristics of the synchronized pumps include: (1) outward pump currents responding to Na-extrusion and inward pump currents responding to K-pumping in are separated; (2) magnitude of the outward pump currents can be up to three times higher than that of the randomly paced pump currents; (3) magnitude ratio of the outward over inward pump currents reveals the 3:2 stoichiometry of the pumps. We, further, gradually increased the field oscillating frequency in a stepwise pattern and kept pump synchronization in each step. We found that the pumps' turnover rate could be modulated up as the field frequency increased. Consequently, the pump currents significantly increased by many fold. In summary, these results show that the catalytic cycle of Na/K pumps can be synchronized and modulated by a well designed oscillating electric field resulting in activation of the pump functions. Springer Science+Business Media, LLC, 2007 Na/K pump nationallicence Synchronization nationallicence Modulation nationallicence Membrane proteins nationallicence Electric field nationallicence Chen Wei Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, 33620, Tampa, FL, USA aut Zhang Zhongsheng Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, 33620, Tampa, FL, USA aut Huang Feiran Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, 33620, Tampa, FL, USA aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 331-339 0145-479X 39:4<331 2007 39 10863 https://doi.org/10.1007/s10863-007-9096-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9096-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Wei Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, 33620, Tampa, FL, USA aut NATIONALLICENCE 700 1- Zhang Zhongsheng Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, 33620, Tampa, FL, USA aut NATIONALLICENCE 700 1- Huang Feiran Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, 33620, Tampa, FL, USA aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 331-339 0145-479X 39:4<331 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer