caa a22 4500 463208544 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10863-007-9089-7 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9089-7 The boxing glove shape of subunit d of the yeast V-ATPase in solution and the importance of disulfide formation for folding of this protein [Elektronische Daten] [Youg Thaker, Manfred Roessle, Gerhard Grüber] The low resolution structure of subunit d (Vma6p) of the Saccharomyces cerevisiae V-ATPase was determined from solution X-ray scattering data. The protein is a boxing glove-shaped molecule consisting of two distinct domains, with a width of about 6.5nm and 3.5nm, respectively. To understand the importance of the N- and C-termini inside the protein, four truncated forms of subunit d (d 11-345, d 38-345, d 1-328 and d 1-298) and mutant subunit d, with a substitution of Cys329 against Ser, were expressed, and only d 11-345, containing all six cysteine residues was soluble. The structural properties of d depends strongly on the presence of a disulfide bond. Changes in response to disulfide formation have been studied by fluorescence- and CD spectroscopy, and biochemical approaches. Cysteins, involved in disulfide bridges, were analyzed by MALDI-TOF mass spectrometry. Finally, the solution structure of subunit d will be discussed in terms of the topological arrangement of the V1VO ATPase. Springer Science+Business Media, LLC, 2007 Vacuolar-type ATPase nationallicence V1VO ATPase nationallicence V1 ATPase nationallicence Vma6p nationallicence Subunit d nationallicence Small angle X-ray scattering nationallicence A1AO ATP synthase nationallicence Subunit C nationallicence Peripheral stalk nationallicence Thaker Youg School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Roessle Manfred European Molecular Biology Laboratory, Hamburg Outstation, EMBL c/o DESY, 22603, Hamburg, Germany aut Grüber Gerhard School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 275-289 0145-479X 39:4<275 2007 39 10863 https://doi.org/10.1007/s10863-007-9089-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9089-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thaker Youg School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 700 1- Roessle Manfred European Molecular Biology Laboratory, Hamburg Outstation, EMBL c/o DESY, 22603, Hamburg, Germany aut NATIONALLICENCE 700 1- Grüber Gerhard School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 275-289 0145-479X 39:4<275 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer