caa a22 4500 463208552 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10863-007-9095-9 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9095-9 Bafilomycin A1 is a potassium ionophore that impairs mitochondrial functions [Elektronische Daten] [Vera Teplova, Anton Tonshin, Pavel Grigoriev, Nils-Erik Saris, Mirja Salkinoja-Salonen] Novel activities of bafilomycin A1, a macrolide antibiotic known as an inhibitor of V-ATPases, were discovered. Bafilomycin A1 induced uptake of potassium ions by energized mitochondria and caused mitochondrial swelling, loss of membrane potential, uncoupling of oxidative phosphorylation, inhibition of the maximal respiration rates, and induced pyridine nucleotide oxidation. The mitochondrial effects provoked by nanomolar concentrations of bafilomycin A1 were connected to its activity as a potent, K+-specific ionophore. The K+ ionophoric activity of bafilomycin A1 was observed also in black lipid membranes, indicating that it was an inherent property of the bafilomycin A1 molecule. It was found that bafilomycin A1 is a K+ carrier but not a channel former. Bafilomycin A1 is the first and currently unique macrolide antibiotic with K+ ionophoric properties. The novel properties of bafilomycin A1 may explain some of the biological effects of this plecomacrolide antibiotic, independent of V-ATPase inhibition. Springer Science+Business Media, LLC, 2007 Macrolide antibiotics nationallicence V-ATPase inhibitor nationallicence Mitochondriotoxic nationallicence K+ influx nationallicence Mitochondrial swelling nationallicence ΔΨm drop nationallicence Uncoupling, black lipid membrane nationallicence Ionophore, potassium carrier nationallicence Teplova Vera Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut Tonshin Anton Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut Grigoriev Pavel Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut Saris Nils-Erik Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut Salkinoja-Salonen Mirja Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 321-329 0145-479X 39:4<321 2007 39 10863 https://doi.org/10.1007/s10863-007-9095-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9095-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Teplova Vera Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Tonshin Anton Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Grigoriev Pavel Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Saris Nils-Erik Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Salkinoja-Salonen Mirja Department of Applied Chemistry and Microbiology, University of Helsinki, Viikki Biocenter 1, POB 56, 00014, Helsinki, Finland aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 321-329 0145-479X 39:4<321 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer