caa a22 4500 463208560 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10863-007-9090-1 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9090-1 Inhibition of anion channels derived from mitochondrial membranes of the rat heart by stilbene disulfonate—DIDS [Elektronische Daten] [Zuzana Tomaskova, Jana Gaburjakova, Anna Brezova, Marta Gaburjakova] The objective of this work was to characterize in more detail the inhibition effect of diisothiocyanatostilbene-2′,2-disulfonic acid (DIDS) on anion channels isolated from the rat heart mitochondria. The channels reconstituted into a planar lipid membrane displayed limited powers of discrimination between anions and cations and the ion conductance measured under asymmetric (250/50mM KCl, cis/trans) and symmetric (150mM KCl) conditions was ∼100pS. DIDS caused a dramatic decrease in the channel activity (IC50 = 11.7 ± 3.1μM) only when it was added to the cis side of a planar lipid membrane. The inhibition was accompanied by the significant prolongation of closings and the shortening of openings within the burst as well as gaps between bursts were prolonged and durations of bursts were reduced. The blockade was complete and irreversible when concentration of DIDS was increased up to 200μM. Our data indicate that DIDS is an allosteric blocker of mitochondrial anion channels and this specific effect could be used as a tool for reliable identification of anion channels on the functional level. Springer Science+Business Media, LLC, 2007 Mitochondria nationallicence Anion channel nationallicence DIDS nationallicence Planar lipid bilayer nationallicence Channel gating kinetics nationallicence Tomaskova Zuzana Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut Gaburjakova Jana Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut Brezova Anna Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut Gaburjakova Marta Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 301-311 0145-479X 39:4<301 2007 39 10863 https://doi.org/10.1007/s10863-007-9090-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9090-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tomaskova Zuzana Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Gaburjakova Jana Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Brezova Anna Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Gaburjakova Marta Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34, Bratislava, Slovak Republic aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 301-311 0145-479X 39:4<301 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer