caa a22 4500 463208579 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10863-007-9092-z doi (NATIONALLICENCE)springer-10.1007/s10863-007-9092-z Effects of glycerol on the thermal dependence of the stability of human erythrocytes [Elektronische Daten] [C. Cunha, L. Arvelos, J. Costa, N. Penha-Silva] Incubation of human blood in saline solution of 0-36% (v/v) ethanol for 30min produces lysis or stabilization of erythrocytes depending on the ethanol concentration. Under less elevated concentrations of ethanol, erythrocytes are present in expanded shapes (R state) that present lower stability and suffer lysis with increase in the ethanol concentration. Under more elevated concentrations of ethanol, erythrocytes are present in contracted shapes (T state) that have higher stability and suffer lysis at even more elevated ethanol concentrations. This work evaluated the effects of glycerol (0 to 2.0M) and temperature (7 to 47°C) on the stability of the R erythrocytes, characterized by the ethanol concentration at the mid-transition point (D 50R ) of the hemolysis curve (D 50R ). D 50R declined sigmoidally with increase in the glycerol concentration or temperature, due to transition of the R to the T state erythrocytes. In 1.5M glycerol, the erythrocytes stability decreased below 32 but increased above 37°C. The combination of temperature, glycerol and ethanol actions generates a critical value of osmotic pressure below which the R state predominates and above which the T state predominates. At 7°C 1.5M glycerol decreased the erythrocytes stability against ethanol but increased the erythrocytes stability against hypotonic shock. Those conditions favor the R state, which has a lower stability against ethanol; however, in the absence of ethanol, glycerol determines less water entrance in the erythrocytes, making more difficult its lysis by hypotonicity. Springer Science+Business Media, LLC, 2007 Membrane stability nationallicence Erythrocytes nationallicence Ethanol nationallicence Glycerol nationallicence Temperature nationallicence Biological thermodynamics nationallicence Cunha C. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut Arvelos L. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut Costa J. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut Penha-Silva N. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 341-347 0145-479X 39:4<341 2007 39 10863 https://doi.org/10.1007/s10863-007-9092-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9092-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cunha C. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut NATIONALLICENCE 700 1- Arvelos L. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut NATIONALLICENCE 700 1- Costa J. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut NATIONALLICENCE 700 1- Penha-Silva N. Institute of Genetics and Biochemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Springer US; http://www.springer-ny.com 39/4(2007-08-01), 341-347 0145-479X 39:4<341 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer