caa a22 4500 463208587 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10863-007-9077-y doi (NATIONALLICENCE)springer-10.1007/s10863-007-9077-y Mitochondrial dysfunction in platelets and hippocampi of senescence-accelerated mice [Elektronische Daten] [Jie Xu, Chun Shi, Qi Li, Jiajia Wu, E. Forster, David Yew] Senescence-accelerated mice (SAM) strains are useful models to understand the mechanisms of age-dependent degeneration. In this study, measurements of the mitochondrial membrane potential (Δψm) of platelets and the Adenosine 5′-triphosphate (ATP) content of hippocampi and platelets were made, and platelet mitochondria were observed in SAMP8 (faster aging mice) and SAMR1 (aging resistant control mice) at 2, 6 and 9 months of age. In addition, an Aβ-induced (Amyloid beta-protein) damage model of platelets was established. After the addition of Aβ, the Δψm of platelets of SAMP8 at 1and 6 months of age were measured. We found that platelet Δψm, and hippocampal and platelet ATP content of SAMP8 mice decreased at a relatively early age compared with SAMR1. The platelets of 6 month-old SAMP8 showed a tolerance to Aβ-induced damages. These results suggest that mitochondrial dysfunction might be one of the mechanisms leading to age-associated degeneration in SAMP mice at an early age and the platelets could serve as a biomarker for detection of mitochondrial function and age related disease. Springer Science+Business Media, LLC, 2007 Aging nationallicence Adenosine 5′-triphosphate nationallicence Mitochondria nationallicence Mitochondrial membrane potential nationallicence Senescence-accelerated mice nationallicence AD : (Alzheimer's disease) nationallicence ADP/O : (Adenosine diphosphate/oxygen) nationallicence ATP : (Adenosine 5′-[tetrahydrogen] triphosphate) nationallicence Aβ : (amyloid beta-protein) nationallicence BDS : (base deactivated silica) nationallicence BP : (Band-pass filter) nationallicence Complex I : (NADH dehydrogenase) nationallicence Complex III : (cytochrome c-oxidoreductase) nationallicence Cu,Zn-SOD 1-3 : (Copper zinc superoxide dismutase) nationallicence DMSO : (Dimethyl sulfoxide) nationallicence DNA : (deoxyribonucleic acid) nationallicence EDTA : (ethylenediaminetetraacetic acid) nationallicence FL : (Fluorescence channel) nationallicence HPLC : (High performance liquid chromatography nationallicence JC-1 : (5,5′,6,6′-tetrachloro-1, 1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide) nationallicence Mn-SOD : (manganese superoxide dismutase) nationallicence NADH : (Nicotinamide adenine dinucleotide, reduced) nationallicence PRP : (platelet-rich plasma) nationallicence PMT : (PhotoMultiplier tube) nationallicence PBS : (Phosphate buffer solution) nationallicence Redox : (oxidation/ reduction reaction) nationallicence SAM : (senescence-accelerated mice) nationallicence SAMP : (strains of accelerated senescence-prone mice) nationallicence SAMP8 : (a substrain of SAMP) nationallicence SAMR : (strains of accelerated senescence-resistant mice) nationallicence SAMR1 : (one of the three substrains of SAMR mice) nationallicence SPSS : (Statistical package for the social sciences) nationallicence TPP : (tetraphenyl-phosphonium) nationallicence 8-OHG : (8-hydroxyguanosine) nationallicence Δψm : (mitochondrial membrane potential) nationallicence Xu Jie Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510080, Guangdong, China aut Shi Chun Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510080, Guangdong, China aut Li Qi Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong aut Wu Jiajia Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510080, Guangdong, China aut Forster E. Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong aut Yew David Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 195-202 0145-479X 39:2<195 2007 39 10863 https://doi.org/10.1007/s10863-007-9077-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9077-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Jie Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510080, Guangdong, China aut NATIONALLICENCE 700 1- Shi Chun Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510080, Guangdong, China aut NATIONALLICENCE 700 1- Li Qi Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong aut NATIONALLICENCE 700 1- Wu Jiajia Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, 510080, Guangdong, China aut NATIONALLICENCE 700 1- Forster E. Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong aut NATIONALLICENCE 700 1- Yew David Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 195-202 0145-479X 39:2<195 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer