caa a22 4500 463208617 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10863-007-9072-3 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9072-3 Resonance Raman spectra of the FMN of the bovine heart NADH: ubiquinone oxidoreductase, the largest membrane protein in the mitochondrial respiratory system [Elektronische Daten] [Hidehisa Sugiyama, Ryohei Nakatsubo, Satoru Yamaguchi, Takashi Ogura, Kyoko Shinzawa-Itoh, Shinya Yoshikawa] The resonance Raman spectra of FMN of the bovine heart NADH: ubiquinone oxidoreductase with the molecular mass of approximately one million dalton were determined by using highly improved enzyme preparation and resonance Raman apparatus. The band positions and the H2O/D2O exchange effect suggest that the N(3)−H group in the ring III of the isoalloxazine moiety is buried inside the protein to increase the vibrational coupling to the C(2)−N(3)-C(4) stretching mode and that the ring I is exposed to the aqueous phase. Springer Science+Business Media, LLC, 2007 NADH: ubiquinone oxidoreductase nationallicence FMN nationallicence Respiratory chain nationallicence Resonance Raman nationallicence FMN : Flavin mononucleotide nationallicence PHBH : para-hydroxybenzoate hydroxylase nationallicence Sugiyama Hidehisa Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut Nakatsubo Ryohei Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut Yamaguchi Satoru Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut Ogura Takashi Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut Shinzawa-Itoh Kyoko Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut Yoshikawa Shinya Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 145-148 0145-479X 39:2<145 2007 39 10863 https://doi.org/10.1007/s10863-007-9072-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9072-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugiyama Hidehisa Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut NATIONALLICENCE 700 1- Nakatsubo Ryohei Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut NATIONALLICENCE 700 1- Yamaguchi Satoru Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut NATIONALLICENCE 700 1- Ogura Takashi Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut NATIONALLICENCE 700 1- Shinzawa-Itoh Kyoko Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut NATIONALLICENCE 700 1- Yoshikawa Shinya Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, 678-1297, Hyogo, Japan aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 145-148 0145-479X 39:2<145 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer