caa a22 4500 463208625 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10863-007-9071-4 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9071-4 Mutations in the Atp1p and Atp3p subunits of yeast ATP synthase differentially affect respiration and fermentation in Saccharomyces cerevisiae [Elektronische Daten] [Brian Francis, Karen White, Peter Thorsness] ATP1-111, a suppressor of the slow-growth phenotype of yme1Δ lacking mitochondrial DNA is due to the substitution of phenylalanine for valine at position 111 of the alpha-subunit of mitochondrial ATP synthase (Atp1p in yeast). The suppressing activity of ATP1-111 requires intact beta (Atp2p) and gamma (Atp3p) subunits of mitochondrial ATP synthase, but not the stator stalk subunits b (Atp4p) and OSCP (Atp5p). ATP1-111 and other similarly suppressing mutations in ATP1 and ATP3 increase the growth rate of wild-type strains lacking mitochondrial DNA. These suppressing mutations decrease the growth rate of yeast containing an intact mitochondrial chromosome on media requiring oxidative phosphorylation, but not when grown on fermentable media. Measurement of chronological aging of yeast in culture reveals that ATP1 and ATP3 suppressor alleles in strains that contain mitochondrial DNA are longer lived than the isogenic wild-type strain. In contrast, the chronological life span of yeast cells lacking mitochondrial DNA and containing these mutations is shorter than that of the isogenic wild-type strain. Spore viability of strains bearing ATP1-111 is reduced compared to wild type, although ATP1-111 enhances the survival of spores that lacked mitochondrial DNA. Springer Science+Business Media, LLC, 2007 ATP synthase nationallicence Yeast mitochondria nationallicence Spore viability nationallicence Chronological aging nationallicence Fermentation nationallicence Respiration nationallicence Francis Brian Department of Molecular Biology, University of Wyoming, 82071, Laramie, Wyoming, USA aut White Karen Department of Molecular Biology, University of Wyoming, 82071, Laramie, Wyoming, USA aut Thorsness Peter Department of Molecular Biology, University of Wyoming, 82071, Laramie, Wyoming, USA aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 127-144 0145-479X 39:2<127 2007 39 10863 https://doi.org/10.1007/s10863-007-9071-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9071-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Francis Brian Department of Molecular Biology, University of Wyoming, 82071, Laramie, Wyoming, USA aut NATIONALLICENCE 700 1- White Karen Department of Molecular Biology, University of Wyoming, 82071, Laramie, Wyoming, USA aut NATIONALLICENCE 700 1- Thorsness Peter Department of Molecular Biology, University of Wyoming, 82071, Laramie, Wyoming, USA aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 127-144 0145-479X 39:2<127 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer