caa a22 4500 463208633 CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10863-007-9073-2 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9073-2 Mitochondrial function plasticity in Acanthamoeba castellanii during growth in batch culture [Elektronische Daten] [Malgorzata Czarna, Francis Sluse, Wieslawa Jarmuszkiewicz] The alterations in mitochondrial bioenergetics during growth in a batch culture of Acanthamoeba castellanii were studied. The capacity of cytochrome pathway-dependent respiration measured in vitro decreased from the intermediary phase, when cell division slowed down. The pattern of the cytochrome pathway capacity changes was paralleled from the intermediary phase by alterations in the amount of total (and reducible) membranous ubiquinone. These changes were accompanied by a decrease in mitochondrial reactive oxygen species production in vitro (when no energy-dissipating system was active), and almost no change in superoxide dismutase activity and protein level, thus indicating an equivalent need for this enzyme in oxidative stress defence in A. castellanii culture. On the other hand, a decrease in the activity and protein level of alternative oxidase and uncoupling protein was observed in vitro, when cells shifted from the exponential growth phase to the stationary phase. It turned out that the contribution of both energy-dissipating systems in the prevention of mitochondrial reactive oxygen species generation in vivo could lead to its constant level throughout the growth cycle of A. castellanii batch culture. Hence, the observed functional plasticity insures survival of high quality cysts of A. castellanii cells. Springer Science+Business Media, LLC, 2007 Acanthamoeba castellanii nationallicence Alternative oxidase nationallicence Cytochrome c oxidase nationallicence Mitochondrial plasticity nationallicence Reactive oxygen species nationallicence Superoxide dismutase nationallicence Ubiquinone nationallicence Uncoupling protein nationallicence AcAOX : alternative oxidase of Acantha- moeba castellanii nationallicence AcUCP : uncoupling protein of Acanthamoeba castellanii nationallicence AOX : alternative oxidase nationallicence BHAM : benzohydroxamate nationallicence BSA : bovine serum albumin nationallicence CAT : carboxyatractyloside nationallicence COX : cytochrome c oxidase nationallicence ΔμH+ : proton electrochemical gradient nationallicence FCCP : carbonyl cyanide p-trifluoromethoxyphenylhydrazone nationallicence FFA : free fatty acid nationallicence LA : linoleic acid nationallicence Q : ubiquinone, coenzyme Q nationallicence ROS : reactive oxygen species nationallicence SOD : superoxide dismutase nationallicence state 3 : phosphorylating respiration (in the presence of ADP) nationallicence state 4 : resting respiration (in the absence of added ADP) nationallicence RCR : respiratory control ratio (state 3/state 4) nationallicence TMPD : N,N,N′N′-tetramethyl-p-phenylenediamine nationallicence UCP : uncoupling protein nationallicence Czarna Malgorzata Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Sluse Francis Laboratory of Bioenergetics and Molecular Physiology, Department of Life Science, Institute of Chemistry B6c, University of Liège, B-4000, Sart-Tilman, Liège, Belgium aut Jarmuszkiewicz Wieslawa Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 149-157 0145-479X 39:2<149 2007 39 10863 https://doi.org/10.1007/s10863-007-9073-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9073-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Czarna Malgorzata Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Sluse Francis Laboratory of Bioenergetics and Molecular Physiology, Department of Life Science, Institute of Chemistry B6c, University of Liège, B-4000, Sart-Tilman, Liège, Belgium aut NATIONALLICENCE 700 1- Jarmuszkiewicz Wieslawa Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 149-157 0145-479X 39:2<149 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer