caa a22 4500 46320865X CHVBK 20180405153133.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10863-007-9074-1 doi (NATIONALLICENCE)springer-10.1007/s10863-007-9074-1 Nitric oxide increases oxidative phosphorylation efficiency [Elektronische Daten] [Pascaline Clerc, Michel Rigoulet, Xavier Leverve, Eric Fontaine] We have studied the effect of nitric oxide (NO) and potassium cyanide (KCN) on oxidative phosphorylation efficiency. Concentrations of NO or KCN that decrease resting oxygen consumption by 10-20% increased oxidative phosphorylation efficiency in mitochondria oxidizing succinate or palmitoyl-L-carnitine, but not in mitochondria oxidizing malate plus glutamate. When compared to malate plus glutamate, succinate or palmitoyl-L-carnitine reduced the redox state of cytochrome oxidase. The relationship between membrane potential and oxygen consumption rates was measured at different degrees of ATP synthesis. The use of malate plus glutamate instead of succinate (that changes the H+/2e− stoichiometry of the respiratory chain) affected the relationship, whereas a change in membrane permeability did not affect it. NO or KCN also affected the relationship, suggesting that they change the H+/2e− stoichiometry of the respiratory chain. We propose that NO may be a natural short-term regulator of mitochondrial physiology that increases oxidative phosphorylation efficiency in a redox-sensitive manner by decreasing the slipping in the proton pumps. Springer Science+Business Media, LLC, 2007 Mitochondria nationallicence Nitric oxide nationallicence Cyanide nationallicence Slipping nationallicence Leak nationallicence Oxidative phosphorylation nationallicence NO : Nitric oxide nationallicence DPTA-NONOate : dipropylenetriamine-NONOate nationallicence EDTA : ethylene-diaminetetraacetic acid nationallicence DNP : 2,4-dinitrophenol nationallicence Pi : inorganic phosphate nationallicence Clerc Pascaline INSERM, U884, F-38041, Grenoble, France aut Rigoulet Michel Institut de Biochimie et de Génetique Cellulaires du CNRS, Université Bordeaux II, Bordeaux, France aut Leverve Xavier INSERM, U884, F-38041, Grenoble, France aut Fontaine Eric INSERM, U884, F-38041, Grenoble, France aut Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 158-166 0145-479X 39:2<158 2007 39 10863 https://doi.org/10.1007/s10863-007-9074-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10863-007-9074-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clerc Pascaline INSERM, U884, F-38041, Grenoble, France aut NATIONALLICENCE 700 1- Rigoulet Michel Institut de Biochimie et de Génetique Cellulaires du CNRS, Université Bordeaux II, Bordeaux, France aut NATIONALLICENCE 700 1- Leverve Xavier INSERM, U884, F-38041, Grenoble, France aut NATIONALLICENCE 700 1- Fontaine Eric INSERM, U884, F-38041, Grenoble, France aut NATIONALLICENCE 773 0- Journal of Bioenergetics and Biomembranes Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 39/2(2007-04-01), 158-166 0145-479X 39:2<158 2007 39 10863 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer