caa a22 4500 463209400 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00412-006-0082-4 doi (NATIONALLICENCE)springer-10.1007/s00412-006-0082-4 Cytogenetic evidence for diversity of two nuclei within a single diplomonad cell of Giardia [Elektronische Daten] [Pavla Tůmová, Klára Hofštetrová, Eva Nohýnková, Ondřej Hovorka, Jiří Král] Giardia intestinalis is an ancient protist that causes the most commonly reported human diarrheal disease of parasitic origin worldwide. An intriguing feature of the Giardia cell is the presence of two morphologically similar nuclei, generally considered equivalent, in spite of the fact that their karyotypes are unknown. We found that within a single cell, the two nuclei differ both in the number and the size of chromosomes and that representatives of two major genetic groups of G. intestinalis possess different karyotypes. Odd chromosome numbers indicate aneuploidy of Giardia nuclei, and their stable occurrence is suggestive of a long-term asexuality. A semi-open type of Giardia mitosis excludes a chromosome interfusion between the nuclei. Differences in karyotype and DNA content, and cell cycle-dependent asynchrony are indicative of diversity of the two Giardia nuclei. Springer-Verlag, 2006 Tůmová Pavla Department of Tropical Medicine, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 12800, Prague 2, Czech Republic aut Hofštetrová Klára Department of Tropical Medicine, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 12800, Prague 2, Czech Republic aut Nohýnková Eva Department of Tropical Medicine, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 12800, Prague 2, Czech Republic aut Hovorka Ondřej Institute of Microbiology, Academy of Sciences, Prague, Czech Republic aut Král Jiří Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic aut Chromosoma Springer-Verlag 116/1(2007-02-01), 65-78 0009-5915 116:1<65 2007 116 412 https://doi.org/10.1007/s00412-006-0082-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-006-0082-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tůmová Pavla Department of Tropical Medicine, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 12800, Prague 2, Czech Republic aut NATIONALLICENCE 700 1- Hofštetrová Klára Department of Tropical Medicine, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 12800, Prague 2, Czech Republic aut NATIONALLICENCE 700 1- Nohýnková Eva Department of Tropical Medicine, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 12800, Prague 2, Czech Republic aut NATIONALLICENCE 700 1- Hovorka Ondřej Institute of Microbiology, Academy of Sciences, Prague, Czech Republic aut NATIONALLICENCE 700 1- Král Jiří Department of Genetics and Microbiology, Charles University in Prague, Prague, Czech Republic aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/1(2007-02-01), 65-78 0009-5915 116:1<65 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer