caa a22 4500 463209478 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00412-007-0117-5 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0117-5 Tandem 41-bp repeats in chicken and Japanese quail genomes: FISH mapping and transcription analysis on lampbrush chromosomes [Elektronische Daten] [Svetlana Deryusheva, Alla Krasikova, Tatiana Kulikova, Elena Gaginskaya] The chromosomal distribution of 41-bp repeats, known as CNM and PO41 repeats in the chicken genome and BglII repeats in the Japanese quail, was analyzed precisely using giant lampbrush chromosomes (LBC) from chicken, Japanese quail, and turkey growing oocytes. The PO41 repeat is conserved in all galliform species, whereas the other repeats are species specific. In chicken and quail, the centromere and subtelomere regions share homologous satellite sequences. RNA polymerase II transcribes the 41-bp repeats in both centromere and subtelomere regions. Ongoing transcription of these repeats was demonstrated by incorporation of BrUTP injected into oocytes at the lampbrush stage. RNA complementary to both strands of CNM and PO41 repeats is present on chicken LBC loops, whereas strand-specific G-rich transcripts are characteristic of BglII repeats in the Japanese quail. The RNA from 41-bp repeats does not undergo cotranscriptional U snRNP-dependent splicing. At the same time, the ribonucleoprotein matrix of transcription units with C-rich RNA of CNM and PO41 repeats was enriched with hnRNP protein K. Potential promoters for satellite transcription are discussed. Springer-Verlag, 2007 Deryusheva Svetlana Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut Krasikova Alla Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut Kulikova Tatiana Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut Gaginskaya Elena Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut Chromosoma Springer-Verlag 116/6(2007-12-01), 519-530 0009-5915 116:6<519 2007 116 412 https://doi.org/10.1007/s00412-007-0117-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0117-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deryusheva Svetlana Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut NATIONALLICENCE 700 1- Krasikova Alla Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut NATIONALLICENCE 700 1- Kulikova Tatiana Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut NATIONALLICENCE 700 1- Gaginskaya Elena Biological Research Institute, Saint-Petersburg State University, Oranienbaumskoie sch. 2, Stary Peterhof, 198504, Saint-Petersburg, Russia aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/6(2007-12-01), 519-530 0009-5915 116:6<519 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer