caa a22 4500 463209486 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00412-007-0118-4 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0118-4 Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting [Elektronische Daten] [Maria Ocampo-Hafalla, Yuki Katou, Katsuhiko Shirahige, Frank Uhlmann] The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres where it provides the cohesive counterforce to bipolar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences transiently split in pre-anaphase cells, in some organisms up to several micrometers. This ‘centromere breathing' presents a paradox, how sister sequences separate where cohesin is most enriched. We now show that in the budding yeast Saccharomyces cerevisiae, cohesin binding diminishes over centromeric sequences that split during breathing. We see no evidence for cohesin translocation to surrounding sequences, suggesting that cohesin is removed from centromeres during breathing. Two pools of cohesin can be distinguished. Cohesin loaded before DNA replication, which has established sister chromatid cohesion, disappears during breathing. In contrast, cohesin loaded after DNA replication is partly retained. As sister centromeres re-associate after transient separation, cohesin is reloaded in a manner independent of the canonical cohesin loader Scc2/Scc4. Efficient centromere re-association requires the cohesion establishment factor Eco1, suggesting that re-establishment of sister chromatid cohesion contributes to the dynamic behaviour of centromeres in mitosis. These findings provide new insights into cohesin behaviour at centromeres. Springer-Verlag, 2007 Ocampo-Hafalla Maria Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK aut Katou Yuki Center for Biological Resources and Informatics, Division of Gene Research, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan aut Shirahige Katsuhiko Center for Biological Resources and Informatics, Division of Gene Research, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan aut Uhlmann Frank Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK aut Chromosoma Springer-Verlag 116/6(2007-12-01), 531-544 0009-5915 116:6<531 2007 116 412 https://doi.org/10.1007/s00412-007-0118-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0118-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ocampo-Hafalla Maria Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK aut NATIONALLICENCE 700 1- Katou Yuki Center for Biological Resources and Informatics, Division of Gene Research, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Shirahige Katsuhiko Center for Biological Resources and Informatics, Division of Gene Research, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Uhlmann Frank Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/6(2007-12-01), 531-544 0009-5915 116:6<531 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer