caa a22 4500 463209494 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00412-007-0119-3 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0119-3 Two X family DNA polymerases, λ and μ, in meiotic tissues of the basidiomycete, Coprinus cinereus [Elektronische Daten] [Aiko Sakamoto, Kazuki Iwabata, Akiyo Koshiyama, Hiroko Sugawara, Takuro Yanai, Yoshihiro Kanai, Ryo Takeuchi, Yoko Daikuhara, Yoichi Takakusagi, Kengo Sakaguchi] The X family DNA polymerases λ (CcPolλ) and μ (CcPolμ) were shown to be expressed during meiotic prophase in the basidiomycete, Coprinus cinereus. These two polymerases are the only members of the X family in the C. cinereus genome. The open reading frame of CcPolλ encoded a predicted product of 800 amino acid residues and that of CcPolμ of 621 amino acid residues. Both CcPolλ and CcPolμ required Mn2+ ions for activity, and both were strongly inhibited by dideoxythymidine triphosphate. Unlike their mammalian counterparts, CcPolλ and CcPolμ had no terminal deoxynucleotidyl transferase activity. Immunostaining analysis revealed that CcPolλ was present at meiotic prophase nuclei in zygotene and pachytene cells, which is the period when homologous chromosomes pair and recombine. CcPolμ was present in a slightly wider range of cell stages, zygotene to diplotene. In analyses using D-loop recombination intermediate substrates, we found that both CcPolλ and CcPolμ could promote primer extension of an invading strand in a D-loop structure. Moreover, both polymerases could fully extend the primer in the D-loop substrate, suggesting that D-loop extension is an activity intrinsic to CcPolλ and CcPolμ. Based on these data, we discuss the possible roles of these polymerases in meiosis. Springer-Verlag, 2007 Sakamoto Aiko Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Iwabata Kazuki Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Koshiyama Akiyo Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Sugawara Hiroko Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Yanai Takuro Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Kanai Yoshihiro Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Takeuchi Ryo Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Daikuhara Yoko Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Takakusagi Yoichi Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Sakaguchi Kengo Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut Chromosoma Springer-Verlag 116/6(2007-12-01), 545-556 0009-5915 116:6<545 2007 116 412 https://doi.org/10.1007/s00412-007-0119-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0119-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakamoto Aiko Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Iwabata Kazuki Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Koshiyama Akiyo Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Sugawara Hiroko Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Yanai Takuro Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Kanai Yoshihiro Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Takeuchi Ryo Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Daikuhara Yoko Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Takakusagi Yoichi Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 700 1- Sakaguchi Kengo Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/6(2007-12-01), 545-556 0009-5915 116:6<545 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer