caa a22 4500 463209532 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s00412-007-0099-3 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0099-3 Evolutionarily conserved, cell type and species-specific higher order chromatin arrangements in interphase nuclei of primates [Elektronische Daten] [Michaela Neusser, Verena Schubel, Andreas Koch, Thomas Cremer, Stefan Müller] Several studies demonstrated a gene-density-correlated radial organization of chromosome territories (CTs) in spherically shaped nuclei of human lymphocytes or lymphoblastoid cells, while CT arrangements in flat-ellipsoidal nuclei of human fibroblasts are affected by both gene density and chromosome size. In the present study, we performed fluorescence in situ hybridization (FISH) experiments to three-dimensionally preserved nuclei (3D-FISH) from human and nonhuman primate cultured lymphoblastoid cells and fibroblasts. We investigated apes, Old, and New World monkeys showing either evolutionarily conserved karyotypes, multiple translocations, fusions, or serial fissions. Our goal was to test whether cell type specific differences of higher order chromatin arrangements are evolutionarily conserved in different primate lineages. Whole genome painting experiments and further detailed analyses of individual chromosomes indicate a gene-density-correlated higher order organization of chromatin in lymphoblastoid cell nuclei of all studied primate species, despite evolutionary chromosome reshuffling. In contrast, in primate fibroblast nuclei evolutionary translocations, fissions and fusions resulted in positional shifts of orthologous chromosome segments, thus arguing against a functional role of chromosome size-dependent spatial chromatin arrangements and for geometrical constraints in flat-ellipsoidal fibroblast nuclei. Notably, in both cell types, regions of rearranged chromosomes with distinct differences in gene density showed polarized arrangements with the more gene-dense segment oriented towards the nuclear interior. Our results indicate that nonrandom breakage and rejoining of preferentially gene-dense chromosomes or chromosome segments may have occurred during evolution. Springer-Verlag, 2007 Neusser Michaela Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut Schubel Verena Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut Koch Andreas Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut Cremer Thomas Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut Müller Stefan Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut Chromosoma Springer-Verlag 116/3(2007-06-01), 307-320 0009-5915 116:3<307 2007 116 412 https://doi.org/10.1007/s00412-007-0099-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0099-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neusser Michaela Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Schubel Verena Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Koch Andreas Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Cremer Thomas Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 700 1- Müller Stefan Department Biology II, Human Genetics, Ludwig-Maximilians-University, Großhaderner Strasse 2, 82152, Planegg-Martinsried, Germany aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/3(2007-06-01), 307-320 0009-5915 116:3<307 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer