caa a22 4500 463209559 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s00412-007-0096-6 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0096-6 A hemicentric inversion in the maize line knobless Tama flint created two sites of centromeric elements and moved the kinetochore-forming region [Elektronische Daten] [Jonathan Lamb, Julie Meyer, James Birchler] A maize line, knobless Tama flint (KTF), was found to contain a version of chromosome 8 with two spatially distinct regions of centromeric elements, one at the original genetic position and the other at a novel location on the long arm. The new site of centromeric elements functions as the kinetochore-forming region resulting in a change of arm length ratio. Examination of fluorescence in situ hybridization markers on chromosome 8 revealed an inversion between the two centromere sites relative to standard maize lines, indicating that this chromosome 8 resulted from a hemicentric inversion with one breakpoint approximately 20centi-McClintocks (cMc) on the long arm (20% of the total arm length from the centromere) and the other in the original cluster of centromere repeats. This inversion moved the kinetochore-forming region but left the remainder of the centromere repeats. In a hybrid between a standard line (Mo17) and KTF, both chromosome 8 homologues were completely synapsed at pachytene despite the inversion. Although the homologous centromeres were not paired, they were always correctly oriented at anaphase and migrated to opposite poles. Additionally, recombination on 8L was severely repressed in the hybrid. Springer-Verlag, 2007 Lamb Jonathan Division of Biological Sciences, University of Missouri-Columbia, 117 Tucker Hall, 65211, Columbia, MO, USA aut Meyer Julie Department of Agriculture, Truman State University, 63501, Kirksville, MO, USA aut Birchler James Division of Biological Sciences, University of Missouri-Columbia, 117 Tucker Hall, 65211, Columbia, MO, USA aut Chromosoma Springer-Verlag 116/3(2007-06-01), 237-247 0009-5915 116:3<237 2007 116 412 https://doi.org/10.1007/s00412-007-0096-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0096-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lamb Jonathan Division of Biological Sciences, University of Missouri-Columbia, 117 Tucker Hall, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Meyer Julie Department of Agriculture, Truman State University, 63501, Kirksville, MO, USA aut NATIONALLICENCE 700 1- Birchler James Division of Biological Sciences, University of Missouri-Columbia, 117 Tucker Hall, 65211, Columbia, MO, USA aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/3(2007-06-01), 237-247 0009-5915 116:3<237 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer