caa a22 4500 463209575 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s00412-007-0102-z doi (NATIONALLICENCE)springer-10.1007/s00412-007-0102-z CENH3 interacts with the centromeric retrotransposon cereba and GC-rich satellites and locates to centromeric substructures in barley [Elektronische Daten] [Andreas Houben, Elizabeth Schroeder-Reiter, Kiyotaka Nagaki, Shuhei Nasuda, Gerhard Wanner, Minoru Murata, Takashi Endo] The chromosomal location of centromere-specific histone H3 (CENH3) is the assembly site for the kinetochore complex of active centromeres. Chromatin immunoprecipitation data indicated that CENH3 interacts in barley with cereba, a centromeric retroelement (CR)-like element conserved among cereal centromeres and barley-specific GC-rich centromeric satellite sequences. Anti-CENH3 signals on extended chromatin fibers always colocalized with the centromeric sequences but did not encompass the entire area covered by such centromeric repeats. This indicates that the CENH3 protein is bound only to a fraction of the centromeric repeats. At mitotic metaphase, CENH3, histone H3, and serine 10 phosphorylated histone H3 predominated within distinct structural subdomains of the centromere, as demonstrated by immunogold labeling for high resolution scanning electron microscopy. Springer-Verlag, 2007 Houben Andreas Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany aut Schroeder-Reiter Elizabeth Department of Biology I, Ludwig-Maximillians-Universität München, Menzinger Straße 67, 80638, Munich, Germany aut Nagaki Kiyotaka Research Institute for Bioresources, Okayama University, Chuo 2-20-1, 710-0046, Kurashiki, Japan aut Nasuda Shuhei Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Wanner Gerhard Department of Biology I, Ludwig-Maximillians-Universität München, Menzinger Straße 67, 80638, Munich, Germany aut Murata Minoru Research Institute for Bioresources, Okayama University, Chuo 2-20-1, 710-0046, Kurashiki, Japan aut Endo Takashi Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Chromosoma Springer-Verlag 116/3(2007-06-01), 275-283 0009-5915 116:3<275 2007 116 412 https://doi.org/10.1007/s00412-007-0102-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0102-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Houben Andreas Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Schroeder-Reiter Elizabeth Department of Biology I, Ludwig-Maximillians-Universität München, Menzinger Straße 67, 80638, Munich, Germany aut NATIONALLICENCE 700 1- Nagaki Kiyotaka Research Institute for Bioresources, Okayama University, Chuo 2-20-1, 710-0046, Kurashiki, Japan aut NATIONALLICENCE 700 1- Nasuda Shuhei Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Wanner Gerhard Department of Biology I, Ludwig-Maximillians-Universität München, Menzinger Straße 67, 80638, Munich, Germany aut NATIONALLICENCE 700 1- Murata Minoru Research Institute for Bioresources, Okayama University, Chuo 2-20-1, 710-0046, Kurashiki, Japan aut NATIONALLICENCE 700 1- Endo Takashi Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/3(2007-06-01), 275-283 0009-5915 116:3<275 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer