caa a22 4500 463209583 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s00412-007-0097-5 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0097-5 Condensin I binds chromatin early in prophase and displays a highly dynamic association with Drosophila mitotic chromosomes [Elektronische Daten] [Raquel Oliveira, Stefan Heidmann, Claudio Sunkel] The condensed state of mitotic chromosomes is crucial for faithful genome segregation. Key factors implicated in the formation of mitotic chromosomes are the condensin I and II complexes. In Drosophila, condensin I appears to play a major role in mitotic chromosome organization. To analyze its dynamic behavior, we expressed Barren, a condensin I non-Structural Maintenance of Chromosomes subunit, as a fully functional enhanced green fluorescent protein (EGFP) fusion protein in the female and followed it during early embryonic divisions. We find that, in Drosophila, Barren-EGFP associates with chromatin early in prophase concomitantly with the initiation of chromosome condensation. Barren-EGFP loading starts at the centromeric region from where it spreads distally reaching maximum accumulation at metaphase/early anaphase. Fluorescence Recovery After Photobleaching analysis indicates that most of the bound protein exchanges rapidly with the cytoplasmic pool during prometaphase/metaphase. Taken together, our results suggest that in Drosophila, condensin I is involved in the initial stages of chromosome condensation. Furthermore, the rapid turnover of Barren-EGFP indicates that the mechanism by which condensin I promotes mitotic chromosome organization is inconsistent with a static scaffold model. Springer-Verlag, 2007 Oliveira Raquel Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180, Porto, Portugal aut Heidmann Stefan Department of Genetics, Bayreuth Center for Molecular Biosciences (BZMB), University of Bayreuth, Bayreuth, Germany aut Sunkel Claudio Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180, Porto, Portugal aut Chromosoma Springer-Verlag 116/3(2007-06-01), 259-274 0009-5915 116:3<259 2007 116 412 https://doi.org/10.1007/s00412-007-0097-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0097-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oliveira Raquel Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180, Porto, Portugal aut NATIONALLICENCE 700 1- Heidmann Stefan Department of Genetics, Bayreuth Center for Molecular Biosciences (BZMB), University of Bayreuth, Bayreuth, Germany aut NATIONALLICENCE 700 1- Sunkel Claudio Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180, Porto, Portugal aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/3(2007-06-01), 259-274 0009-5915 116:3<259 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer