caa a22 4500 463209621 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00412-007-0106-8 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0106-8 Structural differences in centromeric heterochromatin are spatially reconciled on fertilisation in the mouse zygote [Elektronische Daten] [Aline Probst, Fátima Santos, Wolf Reik, Geneviève Almouzni, Wendy Dean] In mammals, paternal and maternal pronuclei undergo profound chromatin reorganisation upon fertilisation. How these events are orchestrated within centromeric regions to ensure proper chromosome segregation in the following cellular divisions is unknown. In this study, we followed the dynamic unfolding of the centromeric regions, i.e. the centric and pericentric satellite repeats, by DNA fluorescent in situ hybridization (FISH) during the first cell cycle up to the two-cell stage. The distinct chromatin from female and male gametes both undergo rapid remodelling and reach a zygotic organisation in which the satellites occupy restricted spatial domains surrounding the nucleolar precursor body. A transition from this zygotic to a somatic cell-like organisation takes place during the two-cell stage. Using 3D immuno-FISH, we find that, whereas maternal pericentric regions are marked with H3K9me3, H4K20me3 and HP1β, paternal ones only showed HP1β marking. Thus, despite different chromatin features, male and female pronuclei organise their centromeric regions in the same way within the nuclei to align chromosomes on the metaphase plate and segregate them appropriately. Our findings highlight the importance of ensuring a proper centromere function while preserving the distinction of parental genome origin during the return to totipotency in the zygote. Springer-Verlag, 2007 Probst Aline Laboratory of Nuclear Dynamics and Nuclear Plasticity, UMR218 CNRS/Institut Curie, 75248, Paris Cedex 05, France aut Santos Fátima Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, CB2 4AT, Cambridge, UK aut Reik Wolf Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, CB2 4AT, Cambridge, UK aut Almouzni Geneviève Laboratory of Nuclear Dynamics and Nuclear Plasticity, UMR218 CNRS/Institut Curie, 75248, Paris Cedex 05, France aut Dean Wendy Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, CB2 4AT, Cambridge, UK aut Chromosoma Springer-Verlag 116/4(2007-08-01), 403-415 0009-5915 116:4<403 2007 116 412 https://doi.org/10.1007/s00412-007-0106-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0106-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Probst Aline Laboratory of Nuclear Dynamics and Nuclear Plasticity, UMR218 CNRS/Institut Curie, 75248, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Santos Fátima Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, CB2 4AT, Cambridge, UK aut NATIONALLICENCE 700 1- Reik Wolf Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, CB2 4AT, Cambridge, UK aut NATIONALLICENCE 700 1- Almouzni Geneviève Laboratory of Nuclear Dynamics and Nuclear Plasticity, UMR218 CNRS/Institut Curie, 75248, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Dean Wendy Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Babraham Research Campus, CB2 4AT, Cambridge, UK aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/4(2007-08-01), 403-415 0009-5915 116:4<403 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer