caa a22 4500 463209699 CHVBK 20180405153136.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s00412-007-0107-7 doi (NATIONALLICENCE)springer-10.1007/s00412-007-0107-7 Characterization of a new SUMO-1 nuclear body (SNB) enriched in pCREB, CBP, c-Jun in neuron-like UR61 cells [Elektronische Daten] [Joaquín Navascués, Rocio Bengoechea, Olga Tapia, José Vaqué, Miguel Lafarga, Maria Berciano] The neuron-like UR61 cell is a stable PC12 subline that contains a mouse N-ras oncogene. Dexamethasone (Dex) treatment induces a neuron-like differentiation, which is associated with neuritogenesis and nuclear expression of the glucocorticoid receptor and c-Jun. In differentiated UR61 cells, small ubiquitin-like modifiers 1 (SUMO-1) is concentrated in a new category of SUMO-1 nuclear bodies (SNBs) distinct from promyelocytic leukemia (PML) bodies by their large size and absence of PML protein. SNBs are 1 to 3μm in diameter and exhibit a fine granular texture by electron microscopy. They are free of splicing factors and transcription foci and show spatial associations with Cajal bodies. In addition to SUMO-1 and the E2-conjugating enzyme Ubc9, which is essential for sumoylation, SNBs concentrate the transcriptional regulators CBP, CREB, and c-Jun. Moreover, transfection experiments demonstrate that SNBs accumulate the active conjugating form of SUMO-1 but not the conjugation defective variant of SUMO-1, supporting that SNBs are sites of sumoylation. Our results suggest that SNBs play a role in the control of the nucleoplasmic concentration of transcription regulators involved in neuroprotection and survival of the UR61 cells. Springer-Verlag, 2007 Navascués Joaquín Division of Gene Regulation and Expression,Wellcome Trust Biocentre, University of Dundee, DD1 5EH, Dundee, UK aut Bengoechea Rocio Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut Tapia Olga Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut Vaqué José Department of Molecular Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut Lafarga Miguel Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut Berciano Maria Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut Chromosoma Springer-Verlag 116/5(2007-10-01), 441-451 0009-5915 116:5<441 2007 116 412 https://doi.org/10.1007/s00412-007-0107-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-007-0107-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Navascués Joaquín Division of Gene Regulation and Expression,Wellcome Trust Biocentre, University of Dundee, DD1 5EH, Dundee, UK aut NATIONALLICENCE 700 1- Bengoechea Rocio Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut NATIONALLICENCE 700 1- Tapia Olga Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut NATIONALLICENCE 700 1- Vaqué José Department of Molecular Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut NATIONALLICENCE 700 1- Lafarga Miguel Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut NATIONALLICENCE 700 1- Berciano Maria Department of Anatomy and Cell Biology, Biomedicine Unit, CSIC, University of Cantabria, Santander, Spain aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/5(2007-10-01), 441-451 0009-5915 116:5<441 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer