caa a22 4500 463209745 CHVBK 20180405153137.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00412-006-0087-z doi (NATIONALLICENCE)springer-10.1007/s00412-006-0087-z The histone chaperone Asf1 at the crossroads of chromatin and DNA checkpoint pathways [Elektronische Daten] [Florence Mousson, Françoise Ochsenbein, Carl Mann] Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shuttled into the nucleus where they are associated with chaperone proteins. Chaperones of histones H3/H4 include CAF-I, the Hir proteins, and Asf1. CAF-I and the Hir proteins function as replication-coupled and replication-independent deposition factors for H3/H4, respectively, whereas Asf1 may play a role in both pathways. In addition to acting as assembly factors, histone chaperones assist nucleosome dissociation from DNA and they may recruit other proteins to chromatin. The past few years have witnessed a notable accumulation of genetic, biochemical, and structural data on Asf1, which motivated this review. We discuss the sequence and structural features of Asf1 before considering its roles in nucleosome assembly/disassembly, the cellular response to DNA damage, and the regulation of gene expression. We emphasize the key role of Asf1 as a central node in a network of partners that place it at the crossroads of chromatin and DNA checkpoint pathways. Springer-Verlag, 2006 Mousson Florence Département de Biologie Joliot-Curie, Service de Biophysique des Fonctions Membranaires, CEA/Saclay, 91191, Gif-sur-Yvette, France aut Ochsenbein Françoise Département de Biologie Joliot-Curie, Service de Biophysique des Fonctions Membranaires, CEA/Saclay, 91191, Gif-sur-Yvette, France aut Mann Carl Département de Biologie Joliot-Curie, Service de Biochimie et de génétique moléculaire, CEA/Saclay, 91191, Gif-sur-Yvette, France aut Chromosoma Springer-Verlag 116/2(2007-04-01), 79-93 0009-5915 116:2<79 2007 116 412 https://doi.org/10.1007/s00412-006-0087-z text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-006-0087-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mousson Florence Département de Biologie Joliot-Curie, Service de Biophysique des Fonctions Membranaires, CEA/Saclay, 91191, Gif-sur-Yvette, France aut NATIONALLICENCE 700 1- Ochsenbein Françoise Département de Biologie Joliot-Curie, Service de Biophysique des Fonctions Membranaires, CEA/Saclay, 91191, Gif-sur-Yvette, France aut NATIONALLICENCE 700 1- Mann Carl Département de Biologie Joliot-Curie, Service de Biochimie et de génétique moléculaire, CEA/Saclay, 91191, Gif-sur-Yvette, France aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/2(2007-04-01), 79-93 0009-5915 116:2<79 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer