caa a22 4500 463209834 CHVBK 20180405153137.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00412-006-0086-0 doi (NATIONALLICENCE)springer-10.1007/s00412-006-0086-0 Probing the W chromosome of the codling moth, Cydia pomonella , with sequences from microdissected sex chromatin [Elektronische Daten] [Iva Fuková, Walther Traut, Magda Vítková, Petr Nguyen, Svatava Kubíčková, František Marec] The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. With fluorescence in situ hybridization (FISH), the euchromatin probe labelled all chromosomes, whereas the W-chromatin DNA proved to be a highly specific W-chromosome painting probe. For sequence analysis, DOP-PCR-generated DNA fragments were cloned, sequenced, and tested by Southern hybridization. We recovered single-copy and low-copy W-specific sequences, a sequence that was located only in the W and the Z chromosome, multi-copy sequences that were enriched in the W chromosome but occurred also elsewhere, and ubiquitous multi-copy sequences. Three of the multi-copy sequences were recognized as derived from hitherto unknown retrotransposons. The results show that our approach is feasible and that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism. The W chromosome has attracted repetitive sequences during evolution but also contains unique sequences. Springer-Verlag, 2006 Fuková Iva Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Traut Walther Institut für Biologie, Universität zu Lübeck, 23538, Lübeck, Germany aut Vítková Magda Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Nguyen Petr Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Kubíčková Svatava Veterinary Research Institute, 621 32, Brno, Czech Republic aut Marec František Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Chromosoma Springer-Verlag 116/2(2007-04-01), 135-145 0009-5915 116:2<135 2007 116 412 https://doi.org/10.1007/s00412-006-0086-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00412-006-0086-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fuková Iva Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Traut Walther Institut für Biologie, Universität zu Lübeck, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Vítková Magda Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Nguyen Petr Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Kubíčková Svatava Veterinary Research Institute, 621 32, Brno, Czech Republic aut NATIONALLICENCE 700 1- Marec František Institute of Entomology, Biology Centre, ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 773 0- Chromosoma Springer-Verlag 116/2(2007-04-01), 135-145 0009-5915 116:2<135 2007 116 412 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer