caa a22 4500 463210360 CHVBK 20180405153138.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s00238-006-0098-2 doi (NATIONALLICENCE)springer-10.1007/s00238-006-0098-2 Expression of epithelial matrix molecules collagen and laminin and corresponding integrins in chronic wounds [Elektronische Daten] [Jutta Liebau, Stephanie Heidrich, Alfred Berger, Mayer Tenenhaus, Hans-Oliver Rennekampff] Re-epithelialization of cutaneous wounds is a coordinated process of proliferation and migration of keratinocytes at the wound edge. The study objective was to identify the differences in epidermal morphology, keratinocyte proliferation and matrix molecules (laminin 1, laminin 5, type IV collagen) and their specific integrin (α3, α6) expression in biopsies of meshed split thickness grafted and chronic wounds. The mean mitotic index of keratinocytes (ratio of cell cycle associated antigen Ki-67 expressing keratinocytes to basal keratinocytes) was highest in chronic wounds (38.7%) compared to acute wounds (22.25%, range 5.7% to 54%). The mean thickness of the hyper-proliferative epithelium at the wound edge of chronic wounds was 0.69mm compared to 0.15mm at the wound margin of split thickness grafted wounds. Both chronic wounds and skin grafted wounds exhibited strong laminin 5 immunoreactivity at the basal side of the epithelium, which extended under the most forward keratinocytes. Laminin 1 and type IV collagen immunoreactivity did not extend to the wound margin in either skin grafted or chronic wounds. In both transplanted skin and chronic wounds, the integrin sub-units α3 and α6 exhibited a strong pericellular immunoreactivity on the leading keratinocytes of the wound margin. Our data demonstrates that the proliferation of keratinocytes and the expression of associated integrins are not impaired in chronic wounds. Springer-Verlag, 2006 Matrix nationallicence Laminin 5 nationallicence Integrins nationallicence Chronic wounds nationallicence Liebau Jutta Department of Plastic Surgery, Fachklinik Hornheide Münster, Münster, Germany aut Heidrich Stephanie Department of Anaesthesiology, Emergency and Intensive Care Medicine, Regionskrankenhaus, Grossburgwedel, Germany aut Berger Alfred INI, Hannover, Germany aut Tenenhaus Mayer Division of Plastic Surgery, Medical Center, University of California San Diego, San Diego, CA, USA aut Rennekampff Hans-Oliver Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Unfallklinik, Schnarrenbergstrasse 95, 72076, Tübingen, Germany aut European Journal of Plastic Surgery Springer-Verlag 29/5(2007-01-01), 235-242 0930-343X 29:5<235 2007 29 238 https://doi.org/10.1007/s00238-006-0098-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00238-006-0098-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liebau Jutta Department of Plastic Surgery, Fachklinik Hornheide Münster, Münster, Germany aut NATIONALLICENCE 700 1- Heidrich Stephanie Department of Anaesthesiology, Emergency and Intensive Care Medicine, Regionskrankenhaus, Grossburgwedel, Germany aut NATIONALLICENCE 700 1- Berger Alfred INI, Hannover, Germany aut NATIONALLICENCE 700 1- Tenenhaus Mayer Division of Plastic Surgery, Medical Center, University of California San Diego, San Diego, CA, USA aut NATIONALLICENCE 700 1- Rennekampff Hans-Oliver Department of Plastic, Hand and Reconstructive Surgery, Burn Center, BG-Unfallklinik, Schnarrenbergstrasse 95, 72076, Tübingen, Germany aut NATIONALLICENCE 773 0- European Journal of Plastic Surgery Springer-Verlag 29/5(2007-01-01), 235-242 0930-343X 29:5<235 2007 29 238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer