caa a22 4500 463210778 CHVBK 20180405153140.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00238-007-0167-1 doi (NATIONALLICENCE)springer-10.1007/s00238-007-0167-1 Influence of tissue nitration on tissue damage with thermal injury [Elektronische Daten] [Hajime Inoue, Miyuki Tomioka, Masanobu Shimokawa, Hiroyuki Nishikawa, Ryoko Kojima, Norio Kumagai] A gas mediator, nitric oxide, is converted into peroxynitrite in the presence of superoxide anion. Peroxynitrite is a potent oxidant, which injures various tissues and organs by nitration of tyrosine residue in protein and enhances the inflammatory response in the prolonged phase. In this study, the authors investigated the relationship between peroxynitrite-mediated tissue nitration and tissue damage with thermal injury using an experimental burn model. The content of nitrotyrosine in the burned tissue significantly increased 1 to 6h after injury. The nitrotyrosine content in the burned ear significantly decreased with 100mg/kg of LNAME administration. Vascular hyperpermeability was also significantly suppressed in the iNOS antibody immunoneutralized mice 6h after injury. There was a positive correlation between the severity of tissue damage, an indicator of which is the increase in the weight of the burned ear along with the development of edema after injury, and the concentration of nitrotyrosine in the wound tissues. Nitrotyrosine-like immune reactants were also diffusely detected in the burned region and the surrounding areas. These results indicate that peroxynitrite is produced in the surrounding burned region and a reaction of nitration of tissue tyrosine is involved with tissue damage at the burn wound. Therefore, to prevent the systemic vascular hyperpermeability and tissue damage in a large area burn or severe burn patients, the administration of NOS inhibitors or radical erasers may be easy to manage generally by inhibition of peroxynitrite formation. Springer-Verlag, 2007 Nitrotyrosine nationallicence Thermal injury nationallicence Tissue damage nationallicence Peroxynitrite nationallicence Inoue Hajime Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut Tomioka Miyuki Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut Shimokawa Masanobu Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut Nishikawa Hiroyuki Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut Kojima Ryoko Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut Kumagai Norio Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut European Journal of Plastic Surgery Springer-Verlag 30/4(2007-12-01), 177-182 0930-343X 30:4<177 2007 30 238 https://doi.org/10.1007/s00238-007-0167-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00238-007-0167-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inoue Hajime Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut NATIONALLICENCE 700 1- Tomioka Miyuki Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut NATIONALLICENCE 700 1- Shimokawa Masanobu Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut NATIONALLICENCE 700 1- Nishikawa Hiroyuki Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut NATIONALLICENCE 700 1- Kojima Ryoko Institute of Advanced Medical Science, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut NATIONALLICENCE 700 1- Kumagai Norio Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, 216-0015, Miyamae, Kawasaki, Japan aut NATIONALLICENCE 773 0- European Journal of Plastic Surgery Springer-Verlag 30/4(2007-12-01), 177-182 0930-343X 30:4<177 2007 30 238 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer