caa a22 4500 46321224X CHVBK 20180405153145.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s00339-007-4144-0 doi (NATIONALLICENCE)springer-10.1007/s00339-007-4144-0 Resolution of λ /10 in fluorescence microscopy using fast single molecule photo-switching [Elektronische Daten] [C. Geisler, A. Schönle, C. von Middendorff, H. Bock, C. Eggeling, A. Egner, S.W. Hell] We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50nm resolution. Due to the fast and asynchronous image acquisition protocol used in these experiments, we were able to reduce acquisition times to ∼2.5min, which is two orders of magnitude lower than in previous implementations. Springer-Verlag, 2007 Geisler C. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Schönle A. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut von Middendorff C. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Bock H. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Eggeling C. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Egner A. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Hell S.W. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut Applied Physics A Springer-Verlag 88/2(2007-08-01), 223-226 0947-8396 88:2<223 2007 88 339 https://doi.org/10.1007/s00339-007-4144-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00339-007-4144-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geisler C. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Schönle A. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- von Middendorff C. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Bock H. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Eggeling C. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Egner A. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Hell S.W. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany aut NATIONALLICENCE 773 0- Applied Physics A Springer-Verlag 88/2(2007-08-01), 223-226 0947-8396 88:2<223 2007 88 339 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer