caa a22 4500 463212762 CHVBK 20180405153146.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00339-007-4265-5 doi (NATIONALLICENCE)springer-10.1007/s00339-007-4265-5 Structural changes of thin films from recombinant spider silk proteins upon post-treatment [Elektronische Daten] [E. Metwalli, U. Slotta, C. Darko, S.V. Roth, T. Scheibel, C.M. Papadakis] Engineering of spider silk proteins offers the possibility to control their molecular sequence and thus their material properties. Spin coating was used to prepare films of engineered spider silk protein derived from the garden spider's (Araneus diadematus) dragline silk protein ADF-4. A conformational transition from α-helix to β-sheet-rich structures upon methanol treatment of the films was detected by external reflection IR spectroscopy. We present direct evidence for this structural transformation using grazing-incidence X-ray diffraction (GIXRD) and small-angle scattering (GISAXS). The protein film structure after the methanol treatment consists mainly of β-sheet polyalanine crystals dispersed in an amorphous protein matrix. The GIXRD intensity profiles show Bragg peaks from β-sheet polyalanine crystallites having an average size of 7.5nm. The non-uniform and large crystal size distributions within the film were explained based on the protein composition. The effect of the chemical nature of the interface on the protein film structure was investigated as well. Springer-Verlag, 2007 Metwalli E. Physikdepartment E13, Technische Universität München, James-Franck-Str. 1, 85747, Garching, Germany aut Slotta U. Department Chemie, Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstr. 4, 85747, Garching, Germany aut Darko C. Physikdepartment E13, Technische Universität München, James-Franck-Str. 1, 85747, Garching, Germany aut Roth S.V. HASYLAB at DESY, Notkestr. 85, 22603, Hamburg, Germany aut Scheibel T. Department Chemie, Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstr. 4, 85747, Garching, Germany aut Papadakis C.M. Physikdepartment E13, Technische Universität München, James-Franck-Str. 1, 85747, Garching, Germany aut Applied Physics A Springer-Verlag 89/3(2007-11-01), 655-661 0947-8396 89:3<655 2007 89 339 https://doi.org/10.1007/s00339-007-4265-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00339-007-4265-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Metwalli E. Physikdepartment E13, Technische Universität München, James-Franck-Str. 1, 85747, Garching, Germany aut NATIONALLICENCE 700 1- Slotta U. Department Chemie, Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstr. 4, 85747, Garching, Germany aut NATIONALLICENCE 700 1- Darko C. Physikdepartment E13, Technische Universität München, James-Franck-Str. 1, 85747, Garching, Germany aut NATIONALLICENCE 700 1- Roth S.V. HASYLAB at DESY, Notkestr. 85, 22603, Hamburg, Germany aut NATIONALLICENCE 700 1- Scheibel T. Department Chemie, Lehrstuhl für Biotechnologie, Technische Universität München, Lichtenbergstr. 4, 85747, Garching, Germany aut NATIONALLICENCE 700 1- Papadakis C.M. Physikdepartment E13, Technische Universität München, James-Franck-Str. 1, 85747, Garching, Germany aut NATIONALLICENCE 773 0- Applied Physics A Springer-Verlag 89/3(2007-11-01), 655-661 0947-8396 89:3<655 2007 89 339 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer