caa a22 4500 463213424 CHVBK 20180405153148.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00339-007-4119-1 doi (NATIONALLICENCE)springer-10.1007/s00339-007-4119-1 Effects of using a metal layer in total internal reflection fluorescence microscopy [Elektronische Daten] [W.T. Tang, E. Chung, Y. Kim, P.T.C. So, C.J.R. Sheppard] This proceeding examines the characteristics of imaging through a metal-coated glass cover slip using total internal reflection fluorescence microscopy (TIRFM). Through back and front focal plane imaging of sub-diffraction-limited fluorescent beads, the experimental characteristics of the emission are compared with theoretical simulations. Furthermore, at the angle at which surface plasmon resonance occurs, we show that the anisotropic emission of the fluorescent beads collected through the metal layer results in a irregular point spread function that has a donut-like structure with multiple concentric rings. Springer-Verlag, 2007 Tang W.T. Computation and Systems Biology, Singapore-MIT Alliance, National University of Singapore, 117576, Singapore, Singapore aut Chung E. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Kim Y. Department of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut So P.T.C. Department of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Sheppard C.J.R. Division of Bioengineering and Department of Diagnostic Radiology, National University of Singapore, 117576, Singapore, Singapore aut Applied Physics A Springer-Verlag 89/2(2007-11-01), 333-335 0947-8396 89:2<333 2007 89 339 https://doi.org/10.1007/s00339-007-4119-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00339-007-4119-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang W.T. Computation and Systems Biology, Singapore-MIT Alliance, National University of Singapore, 117576, Singapore, Singapore aut NATIONALLICENCE 700 1- Chung E. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Kim Y. Department of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- So P.T.C. Department of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Sheppard C.J.R. Division of Bioengineering and Department of Diagnostic Radiology, National University of Singapore, 117576, Singapore, Singapore aut NATIONALLICENCE 773 0- Applied Physics A Springer-Verlag 89/2(2007-11-01), 333-335 0947-8396 89:2<333 2007 89 339 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer