caa a22 4500 463213726 CHVBK 20180405153149.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00339-007-4126-2 doi (NATIONALLICENCE)springer-10.1007/s00339-007-4126-2 Mie scattering and surface plasmon based spectroscopy for the detection of nanoparticle-protein interactions [Elektronische Daten] [G. Doyle, B. Ashall, M. Galvin, M. Berndt, S. Crosbie, D. Zerulla] A spectroscopic study of the kinetic interaction between protein and gold nanoparticles is presented here. Mie scattering in conjunction with excitation of surface plasmons on the surface of the nanoparticles allows access to kinetic information regarding the interactions. The influence of the geometrical shape and size of the nanoparticles regarding the binding of a protein is investigated. Spherical nanoparticles of 60 and 100nm diameters as well as ellipsoidal (rod-shaped) particles of dimension 20 by 60nm for the minor and major axes respectively have been used. By fitting the experimental data with theoretical predictions, the size of the nanoparticle and the distribution in the sample, the shape, the influence of sedimentation and the necessary kinetic parameters have been determined. Simulations were also carried out to predict the Raman spectra of selected molecules for in-depth analysis of the detailed reactions. Springer-Verlag, 2007 Doyle G. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut Ashall B. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut Galvin M. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut Berndt M. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut Crosbie S. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut Zerulla D. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut Applied Physics A Springer-Verlag 89/2(2007-11-01), 351-355 0947-8396 89:2<351 2007 89 339 https://doi.org/10.1007/s00339-007-4126-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00339-007-4126-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doyle G. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut NATIONALLICENCE 700 1- Ashall B. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut NATIONALLICENCE 700 1- Galvin M. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut NATIONALLICENCE 700 1- Berndt M. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut NATIONALLICENCE 700 1- Crosbie S. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut NATIONALLICENCE 700 1- Zerulla D. School of Physics, University College Dublin, UCD, Science Center North, 4, Dublin, Ireland aut NATIONALLICENCE 773 0- Applied Physics A Springer-Verlag 89/2(2007-11-01), 351-355 0947-8396 89:2<351 2007 89 339 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer