caa a22 4500 46321529X CHVBK 20180405153154.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s00339-007-3919-7 doi (NATIONALLICENCE)springer-10.1007/s00339-007-3919-7 Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation [Elektronische Daten] [A. Poghossian, S. Ingebrandt, M.H. Abouzar, M.J. Schöning] The possibilities and limitations of a direct electrical detection of charged macromolecules using a field-effect-based sensor platform is evaluated, mainly focusing on capacitive EIS (electrolyte-insulator-semiconductor) devices. The experimentally obtained results on the detection of DNA immobilisation and hybridisation as well as the monitoring of layer-by-layer adsorbed charged polyelectrolyte (PE) multilayers have been discussed by using two basic possible mechanisms of signal generation, namely the intrinsic charge of the macromolecules and the charge redistribution within the intermolecular spaces or in the multilayer. The effects of the layer-by-layer adsorption conditions (unbuffered and pH buffer solution), and the number and polarity of charged layers on the sensor response have been systematically investigated by means of capacitance-voltage (C-V), constant-capacitance (ConCap) and impedance spectroscopy (IS) methods. Springer-Verlag, 2007 Poghossian A. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany aut Ingebrandt S. Institute of Bio- and Nanosystems, Research Centre Jülich, 52425, Jülich, Germany aut Abouzar M.H. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany aut Schöning M.J. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany aut Applied Physics A Springer-Verlag 87/3(2007-06-01), 517-524 0947-8396 87:3<517 2007 87 339 https://doi.org/10.1007/s00339-007-3919-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00339-007-3919-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poghossian A. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany aut NATIONALLICENCE 700 1- Ingebrandt S. Institute of Bio- and Nanosystems, Research Centre Jülich, 52425, Jülich, Germany aut NATIONALLICENCE 700 1- Abouzar M.H. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany aut NATIONALLICENCE 700 1- Schöning M.J. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany aut NATIONALLICENCE 773 0- Applied Physics A Springer-Verlag 87/3(2007-06-01), 517-524 0947-8396 87:3<517 2007 87 339 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer