caa a22 4500 463216652 CHVBK 20180405153158.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s00226-007-0132-y doi (NATIONALLICENCE)springer-10.1007/s00226-007-0132-y Three-dimensional imaging of a sawn surface: a comparison of confocal microscopy, scanning electron microscopy, and light microscopy combined with serial sectioning [Elektronische Daten] [Lloyd Donaldson, Stig Bardage, Geoffrey Daniel] The three-dimensional structure of a transverse sawn wood surface was investigated using several methods, to compare techniques, and to study the types of deformation in tracheids at the saw cut. A sample of spruce sapwood was cut with a fret saw across the grain. The transverse sawn surface was imaged by confocal microscopy, field emission scanning electron microscopy (FESEM), and by light microscopy combined with serial sectioning and three-dimensional (3D) reconstruction. Both confocal microscopy and FESEM were restricted to visualising the cut surface of the wood. However, serial sectioning was able to reveal the internal structure below the cut surface providing more information on the types of cell deformation present. The wood structure was deformed to depths of more than 600μm below the surface with twisting, crushing and tearing deformations. Near the outer surface, gaps were formed between groups of tracheids where the cell walls had been torn away to form saw dust. The deformation tended to form groups of tracheids that were twisted relative to each other. Latewood was less distorted, forming a dense solid surface compared to the highly fibrous earlywood. Springer-Verlag, 2007 Donaldson Lloyd Cellwall Biotechnology Centre, Scion, Private Bag 3020, Rotorua, New Zealand aut Bardage Stig Wood Ultrastructure Research Centre, Swedish University of Agricultural Science, Uppsala, Sweden aut Daniel Geoffrey Wood Ultrastructure Research Centre, Swedish University of Agricultural Science, Uppsala, Sweden aut Wood Science and Technology Springer-Verlag 41/7(2007-10-01), 551-564 0043-7719 41:7<551 2007 41 226 https://doi.org/10.1007/s00226-007-0132-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00226-007-0132-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donaldson Lloyd Cellwall Biotechnology Centre, Scion, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Bardage Stig Wood Ultrastructure Research Centre, Swedish University of Agricultural Science, Uppsala, Sweden aut NATIONALLICENCE 700 1- Daniel Geoffrey Wood Ultrastructure Research Centre, Swedish University of Agricultural Science, Uppsala, Sweden aut NATIONALLICENCE 773 0- Wood Science and Technology Springer-Verlag 41/7(2007-10-01), 551-564 0043-7719 41:7<551 2007 41 226 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer