caa a22 4500 463217004 CHVBK 20180405153200.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00226-006-0088-3 doi (NATIONALLICENCE)springer-10.1007/s00226-006-0088-3 Dual-axis electron tomography: a new approach for investigating the spatial organization of wood cellulose microfibrils [Elektronische Daten] [Ping Xu, Lloyd Donaldson, Zachary Gergely, L. Staehelin] We have employed dual-axis electron tomography to investigate the 3D organization of cellulose microfibrils in plastic resin-embedded, delignified cell walls of radiata pine early wood. The ∼1nm thick tomographic slices produced in this study provided for a resolution of ∼2nm in the cross-section of the slices throughout the 150nm thick plastic sections. This resolution is sufficient to resolve individual cellulose microfibrils and to map the 3D organization of the cellulose microfibrils within the S2 layer of the secondary cell walls. The individual cellulose microfibrils measure ∼3.2nm in diameter, and appear to consist of a ∼2.2nm unstained core and a ∼0.5nm thick surface layer that is lightly stained. Both individual and clustered cellulose microfibrils are seen surrounded by more heavily stained and irregularly shaped residual lignin and hemicellulose. The tightness of packing of the cellulose microfibrils in the cluster varies along the thickness of the section. These findings demonstrate that dual-axis electron tomography is a technique that can provide new insights into the 3D organization of cellulose microfibrils in plant cell walls. Springer-Verlag, 2006 Xu Ping Wood Quality, Ensis, PO Box 29 237, 8540, Christchurch, New Zealand aut Donaldson Lloyd Cellwall Biotechnology Centre, Scion, Private Bag 3020, Rotorua, New Zealand aut Gergely Zachary Department of Molecular, Cellular and Developmental Biology, UCB 347, University of Colorado, 80309, Boulder, CO, USA aut Staehelin L. Department of Molecular, Cellular and Developmental Biology, UCB 347, University of Colorado, 80309, Boulder, CO, USA aut Wood Science and Technology Springer-Verlag 41/2(2007-02-01), 101-116 0043-7719 41:2<101 2007 41 226 https://doi.org/10.1007/s00226-006-0088-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00226-006-0088-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Ping Wood Quality, Ensis, PO Box 29 237, 8540, Christchurch, New Zealand aut NATIONALLICENCE 700 1- Donaldson Lloyd Cellwall Biotechnology Centre, Scion, Private Bag 3020, Rotorua, New Zealand aut NATIONALLICENCE 700 1- Gergely Zachary Department of Molecular, Cellular and Developmental Biology, UCB 347, University of Colorado, 80309, Boulder, CO, USA aut NATIONALLICENCE 700 1- Staehelin L. Department of Molecular, Cellular and Developmental Biology, UCB 347, University of Colorado, 80309, Boulder, CO, USA aut NATIONALLICENCE 773 0- Wood Science and Technology Springer-Verlag 41/2(2007-02-01), 101-116 0043-7719 41:2<101 2007 41 226 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer